摘要
目的了解临床血液标本中分离的18株弯曲菌的菌种类型与特征。方法按常规生化实验、16S rRNA基因和rop B基因测序技术对分离株进行种型鉴定,结合鉴定结果、生长属性和临床资料分析其病原学特征。结果 18株血流感染的病原菌均为革兰阴性逗点样弯曲或螺旋样弯曲的弧形菌。经16S rRNA基因和rop B基因测序分析,共有13株胎儿弯曲菌龟亚种、1株胎儿弯曲菌胎儿亚种,2株空肠弯曲菌空肠亚种,1株大肠弯曲菌和1株海鸥弯曲菌。临床资料显示,弯曲菌血流感染主要对象为新生儿和有基础性疾病患者(如肝炎、糖尿病、高血压),大多不伴随腹泻症状。体外存活实验显示,胎儿弯曲菌相对于空肠弯曲菌、大肠弯曲菌等具有较强的生存能力,其在含血液的血培养瓶中可存活一年以上。结论胎儿弯曲菌龟亚种可能是我国弯曲菌血流感染的主要菌型。
Objective To identify and characterize 18 strains of Campylobacter isolated from blood samples. Methods The types and subspecies of 18 Campylobacter strains were identified by routine biochemical tests and gene sequencing of 16 S rRNA and rop B. The etiological characteristics were studied by the results of identification,clinical data,type or subspecies and the bacterial growing property. Results All the 18 strains were identified as Gram-negative bacteria with comma-like or spiral curve,including 13 strains of C. fetus subsp. testudinum,1 strain of C. fetus subsp. fetus,2 strains of C. jejuni subsp. jejuni,1 strain of C. coli and C. lari. The clinical data showed that the susceptible individuals were newborn and immunocompromised patients with underlying diseases（ diabetes,hypertension,hepatitis,etc.）. Most of the infected patients did not complicate with diarrheas in the week before onset of disease.The survival tests in vitro showed that the viability of C. fetus subsp. testudinum remained stronger than that of C. jejuni and C. coli.Conclusion C. fetus subsp. testudinum may be the main pathogen of Campylobacter bloodstream infection in China.
出处
《临床检验杂志》
CAS
CSCD
2015年第2期88-93,共6页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(31300004)
广东省中医院朝阳人才基金
关键词
弯曲菌
血流感染
病原学
Campylobacter
bloodstream infection
etiology
