摘要
为解决普鲁兰多糖发酵液色素和蛋白脱除过程中多糖易降解、有机试剂用量大、重复次数多等问题,本论文采用D4020树脂、硅藻土、凹凸棒土等三种吸附剂,同时脱除发酵液中的色素和蛋白。通过考察时间、温度、p H、用量等因素对色素脱除率、蛋白脱除率和多糖保留率的影响,比较三种吸附剂的吸附条件和吸附效果,得出三种吸附剂对色素和蛋白均有较好的去除效果,其中硅藻土的去除效果最好。在单因素实验的基础上对硅藻土进行了正交实验,得到硅藻土的最优吸附条件为:时间60min,温度35℃,发酵液p H3.2,吸附剂用量1.6%,在该条件下脱色率为80.17%,脱蛋白率为76.28%,多糖保留率为89.82%。
There are many problems in decolorization and deproteinization of pull@an from fermentation broth, such as large toxic organic reagents usage, high repetition times and easy polysaccharide degradation etc.To solve these issues,study for the decolorization and deproteinization of pullulan was developed through static adsorption tests of D4020 macroporous resins, diatomite and attapulgite. This paper investigated how the ratios of decolorization,deproteinization and polysaccharide recovery changed along with the adjustments of adsorption time,temperature,pH and dosage.The results showed that these three kinds of adsorbents all performed well in adsorbing the impurities, and d iatomite was the most suitable one.Based on the single factor experiment,diatomite was then chosen for the orthogonal experiment.The results showed that the optimal processing conditions were as follows：6Omin adsorption time, 35℃adsorption temperature, pH value 3.2 and 1.6% diatomite. Under such conditions,the decolorization ratio was 80.17%, the deproteinization ratio was 76.28% and the polysaccharide recovery ratio was 89.82%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第7期138-143,共6页
Science and Technology of Food Industry
基金
国家863计划项目(2012AA021505)
关键词
吸附
脱色
脱蛋白
普鲁兰多糖
发酵液
adsorption
decolorization
deproteinization
pullulan
fermentation broth
