摘要
研究确定了葡萄酒中可能添加的天然色素种类是花青素类物质,并建立了葡萄酒中花色苷的检测方法,样品经SPE提取后高效液相色谱荧光法进行定性、定量分析。确定了葡萄酒中6种主要呈色物质:锦葵素-3-葡萄糖苷;锦葵素-3-乙酰葡萄糖苷;锦葵素-3-p-香豆酰葡萄糖苷;芍药素-3-葡萄糖苷;飞燕草素-3-葡萄糖苷;矮牵牛色素-3-葡萄糖苷,比例分别为5∶1.5∶1∶0.5∶0.4∶0.4,当酒中原有花色苷种类和比例出现剧烈变化时,即可判定添加了外源花色苷,方法可以有效判定酒中是否添加外源花色苷。
Based on the previous study, natural pigment added to wine were anthocyanins.A method of determining anthocyanins in wine was established.The qualitative and quantitative analysis was investigated by HPLC.6 major anthocyanins in wine were determined ： Mv-3-gl, mv-3-gl-Ac, mv-3-gl-cm, pn-3-gl, df-3-gl and pt-3-gI.Ratio was 5：1.5：1：0.5：0.4：0.4.Therefore, when the contents and proportion were varied dramatically, which showed that natural pigment was applicated into wine.This method was effective in distinguishing whether the anthocyanins added in wine.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第7期290-294,303,共5页
Science and Technology of Food Industry
关键词
高效液相色谱法
鉴别检测
天然色素
high performance liquid chromatography
distinguishing and detection
natural pigment
