摘要
对免疫磁球捕获-PCR（IMS-PCR）检测牛奶中金黄色葡萄球菌进行了初步研究。优化了免疫磁球制备参数,确定了金黄色葡萄球菌免疫磁球对目标菌的结合时间。研究结果显示,当纯菌浓度为101~104CFU/m L水平时,金黄色葡萄球菌免疫磁球对目标菌的捕获率大于80%。通过对目标菌和非目标菌的检测,IMS-PCR检测方法显示了很强的特异性;在纯培养、无需增菌情况,IMS-PCR检测方法检测限为104CFU/m L;牛奶中的金黄色葡萄球菌经磁分离后增菌2h用PCR检测,可检测出104CFU/m L的金黄色葡萄球菌。
The present study investigated the use of immunomagnetic separation （IMS）capture as an additional concentration step before PCR in the detection and identification of Staphylococcus aureus（S.aureus） from milk. The optimum technological parameters of the IMS system and the coupling time of immunocapture reactions were investigated and evaluated.The capture efficiencies of the immunomagnetic beads were above 80% for S.aureus in PBS at a bacterial concentration of 10^1-10^4CFU/mL. S.aureus could be detected in samples that were otherwise negative by IMS-PCR. The IMS-PCR method showed a detection threshold corresponding to 10^4CFU/mL in S.aureus-spiked PBS.ln the case of S.aureus-spiked milk, the minimum value of detection was 10^4CFU/mL after 2h enrichment.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第7期304-307,313,共5页
Science and Technology of Food Industry
基金
北京市优秀人才培养资助项目(2012D002022000001)
