摘要
目的：建立测定去甲斑蝥素齐墩果酸复合脂质体包封率的方法。方法：采用反相高效液相色谱法。色谱柱为Thermo Sycronis C18,流动相为磷酸水溶液（p H=3.2）-甲醇（梯度洗脱）,流速为1.0~1.7 ml/min,检测波长为210 nm,柱温为30℃,进样量为50μl。结果：去甲斑蝥素、齐墩果酸的质量浓度分别在20~300、10~150μg/ml范围内与峰面积呈良好线性关系（r=0.999 8、0.999 7）;精密度、稳定性、重复性试验的RSD≤5.89%;去甲斑蝥素的平均回收率为99.15%~100.11%,RSD≤3.20%（n=5）,齐墩果酸的平均回收率为99.22%~100.30%,RSD≤3.34%（n=5）;去甲斑蝥素、齐墩果酸平均包封率分别为（48.01±2.34）%、（87.31±0.56）%（n=5）。结论：该方法法简便、快速、可靠,可用于去甲斑蝥素齐墩果酸复合脂质体包封率的测定。
OBJECTIVE：To establish a method for the determination of encapsulation efficiency of Norcantharidin and oleanolic acid liposome. METHODS：RP-HPLC method was adopted. The column was Thermo Sycronis C18 and the mobile phase was phosphoric acid（p H=3.2）-methanol（gradient elution） with the flow rate of 1.0-1.7 ml/min,the detection wavelength was 210 nm,the column temperature was 30 ℃,the volume was 50 μl. RESULTS：There was a good linear relationship between the quality concentration of norcantharidin and the peak area in the range of 20-300 μg/ml（r=0.999 8）and oleanolic acid was in the range of 10-150 μg/ml（r=0.999 7）;the RSDs of precision,stability and repeatability tests was no more than 5.89%;the average recovery of oleanolic acid was in the range of 99.15%-100.11%（RSD≤3.20%,n=5）,that of norcantharidin was 99.22%-100.30%（RSD≤3.34%,n=5）;the average encapsulation efficiency of norcantharidin and oleanolic acid were（48.01±2.34）%,（87.31±0.56）%（n=5）,respectively. CONCLUSIONS：The method is simple,fast and reliable to determine the encapsulation efficiency of Norcantharidin and oleanolic acid liposome.
出处
《中国药房》
CAS
北大核心
2015年第9期1238-1240,共3页
China Pharmacy
基金
重庆市自然科学基金资助项目(No.CSTC
2010BB5347)
重庆市卫生局中医药科技项目(No.2011-2-161)
