摘要
谷胱甘肽过氧化物酶4(GPx4)是一类抗氧化酶,能够减少脂质氢过氧化物、氢过氧化物和有机氢过氧化物.从日本七鳃鳗中克隆得到了GPx4(LjGPx4)编码区和基因组DNA序列,揭示了其基因组特征为含有6个外显子结构和5个内含子结构,并将日本七鳃鳗GPx4序列同其他脊椎动物进行了同源序列比对.使用实时定量PCR分析了LjGPx4在日本七鳃鳗中的组织分布.脂多糖(LPS)体内刺激七鳃鳗后发现,LjGPx4mRNA在白细胞中表达升高.日本七鳃鳗GPx4具有高度保守的基因序列和蛋白质结构,并参与抗菌免疫应答,为抗氧化剂酶在无颌脊椎动物的先天免疫反应中发挥至关重要的作用提供了实验依据.
The antioxidant enzyme,glutathione peroxidase 4（GPx4）,is capable of reducing complex lipid hydroperoxides,hydrogen peroxide and organic hydroperoxides.In this study,we cloned and characterized GPx4-coding（designated LjGPx4）and genomic DNA sequences from Arctic lamprey Lampetra japonica.Genomic characterization revealed a six exon-five intron structure.Additionally,LjGPx4 shares high sequence homology with GPx4 of jawed vertebrates.Real-time quantitative PCR revealed wide distribution of LjGPx4 in lamprey tissues.Furthermore,upon in vivo stimulation of lampreys with lipopolysaccharide（LPS）,LjGPx4 mRNA was upregulated in leukocytes.The highly conserved genomic and protein structures of LjGPx4,together with its involvement in the antibacterial immune response of lamprey,provide evidence for the evolutionary existence of this antioxidant enzyme playing a vital role in innate immune responses in jawless vertebrates.
出处
《辽宁师范大学学报（自然科学版）》
CAS
2015年第1期117-124,共8页
Journal of Liaoning Normal University:Natural Science Edition
基金
国家自然科学基金项目(21102067
31170353)
