摘要
本试验旨在研究不同磷酸脲添加量对羔羊血清和肌肉钙(Ca)、磷(P)含量及血清相关激素含量的影响。选用80只F1代杜寒杂交羔羊,随机分为5组,每组8个重复,每个重复2只。分别在各组精料中添加0、1.0%、2.0%、4.0%和8.0%的磷酸脲以替代部分豆粕等蛋白质饲料。预试期为12 d,正试期为60 d。预试期内饲喂精料和粗料比为1∶1,自由采食。正试期内肉羊精料和粗料采食量比为1∶1,各组实际采食饲粮中的磷酸脲含量分别为0(对照组)、0.5%(0.5%组)、1.0%(1.0%组)、2.0%(2.0%组)和4.0%(4.0%组)。每组选取8只羊在1(预试期开始当天)、13和73 d晨饲前采集血液,测定1、13和73 d血清钙、磷含量及1和13 d血清甲状旁腺素(PTH)、降钙素(CT)、25-羟基维生素D(25-OH-VD)含量;73 d每组选取6只羊屠宰,取背腰最长肌进行肌肉中钙、磷含量的测定。结果表明:1)随饲粮中磷酸脲添加量的增加,血清钙含量降低,磷含量增加。其中,血清钙含量在1和73 d时各组间差异不显著(P>0.05),但在13 d时4.0%组显著低于其他各组(P<0.05)。血清磷含量在1 d时各组间差异不显著(P>0.05);13 d时4.0%组显著高于对照组、0.5%组和2.0%组(P<0.05);73 d时4.0%组显著高于对照组和0.5%组(P<0.05),对照组显著低于1.0%组、2.0%组、4.0%组(P<0.05)。2)各组背腰最长肌中的钙、磷含量差异不显著(P>0.05)。3)血清中各组间的PTH、25-OH-VD含量差异不显著(P>0.05);13 d时,1.0%组和2.0%组的血清CT含量显著低于对照组和4.0%组(P<0.05)。由此可见,磷酸脲的添加可使得血清和肌肉中钙含量降低、磷含量增加,对血清PTH和25-OH-VD含量无显著影响,但会引起血清CT含量的显著变化;饲粮添加4.0%的磷酸脲会引起羔羊增重受到抑制。
This experiment was conducted to study the effects of different levels of urea phosphate supplemen-tation on calcium （ Ca） and phosphorus （ P） contents in serum and muscle and related hormone contents in ser-um of lambs. Eighty Dorper＆#215;thin-tailed Han F1 crossbred lambs were randomly divided into 5 groups with 8 replicates per group and 2 lambs per replicate. Soybean meal and some other protein feeds were replaced by 0, 1.0%, 4.0% and 8.0% of urea phosphate in concentrate, respectively. During the 12-day adaptation period, the ratio of concentrate to roughage was 1∶1, and lambs were fed ad libitum; during the 60-day experimental period, the intake of ratio of concentrate to roughage was 1∶1, and the actually intake of urea phosphate levels were 0 （control group）, 0.5% （0.5% group）, 1.0% （1.0% group）, 2.0% （2.0% group） and 4.0% （4.0%group） , respectively. On 1 （ the starting day of adaptation period） , 13 and 73 days, blood samples were col-lected from 8 lambs of each group before morning feeding to determine the contents of Ca, P （ 1, 13 and 73 d） parathyroid hormone （ PTH） , calcitonin （ CT） and 25-hydroxy vitamin D （ 25-OH-VD） （ 1 and 13 d） . On 73 days, six lambs of each group were slaughtered and the longissimus dorsi was sampled to analyze the contents of Ca and P. The results showed as follows： 1 ） in serum, Ca content decreased and P content in-creased with the increasing level of urea phosphate. Serum Ca content showed no significant difference on 1 and 73 days among groups （P〉0.05）, while that of 4.0% group was significantly lower than that of the other groups on 13 days （ P〈0.05） . No significant difference was found in serum P content on 1 day （ P〉0.05） . Se-rum P content of 4.0% group was significantly higher than that of control, 1.0%, and 2.0% groups on 13 days （ P〈0.05） . On 73 days, serum P content of 4.0% group was significantly higher than that of control and 0.5%groups （ P〈0.05） , and control group was significantly lower than that of 1.0%, 2.0%, and 4.0% groups （ P〈0.05） . 2） There were no significant differences of Ca and P contents in longissimus dorsi among groups （ P〉0.05） . 3 ） The differences among the contents of PTH and 25-OH-VD in serum were not significant （ P〉0.05） . The content of CT in 1. 0% and 2. 0% groups was significantly lower than that of control and 4. 0%groups on 13 days （P〈0.05）. In conclusion, Ca content decreases while P content increases in serum and muscle with the supplementation of urea phosphate. Serum of PTH and 25-OH-VD contents are not affected by urea phosphate. The supplementation of urea phosphate can significantly change serum CT content. The supple-mentation of 4.0% of urea phosphate inhibits the increase in weight of lambs.
出处
《动物营养学报》
CAS
CSCD
北大核心
2015年第3期845-852,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
农产品质量安全专项资金(2014)
牧区饲草饲料资源开发利用技术研究与示范(20120304202)
关键词
磷酸脲
羔羊
钙
磷
非蛋白氮
urea phosphate
lambs
calcium
phosphorus
non-protein nitrogen
