摘要
研究异紫堇碱(isocorydine,ICD)半合成衍生物8-氨基-异紫堇碱(8-NH2-ICD)的抗菌及抑制肿瘤生长作用。用琼脂扩散法和最低抑菌浓度测定法测定8-NH2-ICD的抑菌作用,用MTT法测定该化合物对细胞增殖的抑制作用。不同浓度的8-NH2-ICD对7种常见致病菌具有明显抑制作用,其中对伤寒沙门氏菌和大肠埃希菌的抑制作用显著高于其它菌,8-NH2-ICD对产气杆菌的抑制作用相对较弱。8-NH2-ICD对9种肿瘤细胞系生长抑制程度不同,对胃腺癌细胞MGC803和胶质瘤细胞M059K的增殖具有较强的抑制作用,对人正常细胞生长抑制作用较弱。8-NH2-ICD具有较强的抑制病原菌和抑制肿瘤细胞生长的作用。
To investigate the bacteriostasis activity and tumour inhibitory effect of semi-synthetic derivate of isocorydine 8-amino-isocorydine（8-NH2-ICD）. The bacteriostasis activities of alkaloid in vitro were tested with agar diffusion method and minimal inhibitory concentrations（MIC）.Inhibitory activity on proliferation of tumour cells with treated by 8-NH2-ICD were measured by MTT assay. The results show there is a dramatic bacteriostasis activity on Salmonella typhi and Escherichia coli, and relatively mild effect on Enterobacter aerogenes. MGC803 cell and M059 K cell proliferation effect were dramatically inhibited by 8-NH2-ICD in vitro. The anti-tumour activity was gradually enhanced with the alkaloid variety of dose. However, 8-NH2-ICD have been demonstrated with low toxity to normal fibroblasts MRC-5 till concentration up to 200mol/L. To our knowledge, this is the first time that 8-NH2-ICD has anticancer and antibacterial activity is reported.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第1期79-83,共5页
Journal of Food Science and Biotechnology
基金
中央高校基本科研业务费专项资金项目(31920130036)
西北民族大学科研启动费项目(xbmuyjrc201120)
西北民族大学2014年本科生科研创新项目(URIP14287)
2014年国家级大学生创新创业训练项目(201410742058)
西北民族大学医学院校级科研团队项目(xbmuyjrc201120)
