摘要
以10个葡萄柚品种种子萌发的无菌苗叶片为外植体,研究葡萄柚的离体再生体系。结果表明：在MS＋1.0 mg/L 2,4-D＋6%蔗糖培养基上,8个葡萄柚品种能诱导出愈伤组织,其中“星路比”品种诱导率最高为46.67%,将诱导的愈伤组织转移至相同培养基中可继代增殖;“帕利斯”、“星路比”、“泰国种”3个品种的叶愈伤组织接种于MS＋3.0 mg/L 6-BA分化培养基上可分化出丛生芽,这些丛生芽在MS＋0.2 mg/L 6-BA培养基上平均可增殖4~6个芽;丛生芽在1/2 MS＋1.0 mg/L IBA生根培养基上可诱导出不定根。炼苗后,田间移栽成活率为78.18%。为后续葡萄柚良种选育及遗传转化体系的建立奠定基础。
The leaves of aseptic seedlings from the seeds of ten grapefruit varieties as explants were used to establish the in vitro regeneration system of grapefruit. The results showed that the callus of eight grapefruit varieties were observed in the medium MS＋1.0 mg/L 2,4-D＋6% sugar, and ＂Star Ruby＂ had the highest callus induction frequency, which is 46.67%. The multiplication medium of the callus was the same medium. The medium of the differentiation of adventitious buds of three grapefruit varieties ＂ Perlist＂ ＂ Star Ruby＂ and＂Thailand＂ was MS＋3.0 mg/L 6-BA. The medium for multiplication of the regeneration buds was MS＋0.2 mg/L 6-BA, and the rooting medium was 1/2 MS ＋1.0 mg/L IBA. The survival rate of regenerative plant was 78.18%. In order to provide useful references for the breeding and the application of genetic transformation technique in grapefruit.
出处
《热带作物学报》
CSCD
北大核心
2015年第3期487-492,共6页
Chinese Journal of Tropical Crops
基金
云南省教育厅重点项目(No.2014C006Z)
国家林业局948项目(No.2011-4-45)
关键词
葡萄柚
再生植株
愈伤组织
不定芽
Citrus paradisi Macf.
Regenerative plant
Callus
Adventitious bud
