摘要
为了研究硒对农作物胁迫的机理,本试验以小白菜（Brassica rapa chinensis）幼苗为材料,研究了亚硒酸钠（Na2Se O3）处理下,根中内源信号分子一氧化氮（NO）和硫化氢（H2S）的变化与BrRboh G1和BrRboh G2表达的关系。结果表明：0.03-0.46 mmol/L的Na2Se O3显著抑制根的生长,并呈现浓度效应;随着Na2Se O3处理浓度的升高,内源NO含量逐渐上升,而内源H2S逐渐下降,两者呈显著负相关;添加NO合成抑制剂（L-NMMA和Tungstate）或NO清除剂（c PTIO）能够显著抑制Na2Se O3对内源H2S的诱导作用;Na2Se O3处理能够显著诱导根内BrRboh G1和BrRboh G2的表达,分别添加L-NMMA、Tungstate、c PTIO、H2S供体Na HS均能显著抑制Na2Se O3对上述两基因表达的诱导作用。说明Na2Se O3处理下,内源NO介导了Na2Se O3对BrRboh G1和BrRboh G2的诱导表达,而H2S则可能抑制了两个基因的表达;NO也可能作用于H2S信号的上游。
Selenium（ Se） has been becoming an emergent pollutant in the agricultural environment. In order to explore the mechanism of Se-induced stress responses in crops,the present study investigated the effect of Na2 Se O3treatment on two important endogenous signalling molecules,hydrogen sulfide（ H2S） and nitric oxide（ NO）,on the roots of Brassica rapa and the regulation of the expression of BrRboh G1 and BrRboh G2 by H2 S and NO. Results were achieved as follows. The root growth was significantly inhibited by Na2 Se O3varying from 0. 03 mmol / L to 0. 46 mmol / L in a dose-dependent manner. Compared to the control group, the root elongation was decreased by almost 60 % in the treatment of Na2 Se O3at 0. 06 mmol / L. Treatment with Na2 Se O3resulted in the increase in endogenous NO content and the decrease in endogenous H2 S content in root in dose-dependent manners. The correlation analysis suggested that there was a significantly negative correlation between NO and H2 S in root with Na2 Se O3treatment. The inhibition of H2 S induced by 0. 06 mmol / L Na2 Se O3could be significantly attenuated by the addition of both NO synthesized inhibitors（L-NMMA and Tungstate） and NO scavenger（ c PTIO）. The expressions of BrRboh G1 and BrRboh G2 could be induced significantly by Na2 Se O3at 0. 06 mmol / L,which could be inhibited by the addition ofL-NMMA,Tungstate,c PTIO,and H2 S donor sodium hydrosulfide（ Na HS）,respectively. In conclusion,both endogenous NO and H2 S are probably involved in the regulation of the expressions of BrRboh G1 and BrRboh G2 in the root of B. rapa under Na2 Se O3stress. The endogenous NO mediated Na2 Se O3-induced expressions of BrRboh G1 and BrRboh G2 while the endogenous H2 S might inhibit the expressions of BrRboh G1 and BrRboh G2 in the roots of B. rapa under Na2 Se O3stress.And NO might have functioned the upstream of H2 S in the above process.
出处
《江苏农业学报》
CSCD
北大核心
2015年第1期149-157,共9页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31401857)
江苏省农业科技自主创新基金项目[CX(13)5052]
