摘要
目的建立一种高效、简便的分离纯化小鼠精原细胞的方法。方法利用胶原酶IV／脱氧核糖核酸酶I（DNaseI）／分散酶（dispase）组合酶消化法和差异性贴壁原理，从新生小鼠睾丸中分离、纯化精原细胞。经细胞形态学观察、视黄酸激活基因8（Stra8）免疫化学鉴定精原细胞并进行纯度分析。结果分离到的细胞经Stra8检测呈阳性，精原细胞纯度可达86％。结论该方法可高效地分离和纯化小鼠精原细胞。
Objective To establish a simple and efficient method for separation and purification of mouse spermatogonia. Methods Spermatogonia were isolated and purified from neonatal mouse testis using collagenase IV/DNase I/dispase combination enzymatic digestion and differential velocity of cell adherence method. Isolated spermatogonia were analyzed by morphology and stra8 immunochemistry. Results The isolated spermatogonia were stra8 positive and cell purity was as high as 86%. Condusion This method is efficient for separation and purification of mouse spermatogonia
出处
《实验动物与比较医学》
CAS
2015年第1期42-45,共4页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金资助项目（编号81270760）
关键词
小鼠
精原细胞
分离
纯化
Mouse
Spermatogonia
Isolation
Purification
