摘要
本文以EMS诱变处理野生型拟南芥Col-0获得的M_2代种子为试验材料,通过在不含钼(-Mo)和含170 nmol·L^(-1)钼(+Mo)的培养基上进行反复筛选培养,根据拟南芥根系在2种培养基上的生长差异筛选出31株Mo敏感变异株。并对变异株2-4的表型特征及鲜重和Mo含量进行了分析。结果显示,在-Mo培养基中变异株2-4的生长明显受到抑制,且叶鲜重、根鲜重和Mo含量均低于野生型。而在+Mo培养基中变异株2-4叶鲜重、根鲜重与野生型的差异不明显,且都高于在-Mo培养基中的。随着钼含量的增加,变异株2-4矮小、叶片失绿和叶缘卷曲的症状不断恢复。当钼含量达到170 nmol·L^(-1)时,变异株2-4的生长情况与野生型相比没有明显差异。这说明筛选出的2-4植株是一种Mo敏感型变异株。
In this study, M2 generation seeds from wild-type Arabidopsis thaliana Col-0 treated with ethylmeth- ane sulfonate （EMS） were used as experimental materials, which were cultured on the no-molybdenum （-Mo） and 170 nmol＇L＂1 molybdenum （＋Mo） media for repeated screening. According to the growth status of roots in two media, 31 plantlets ofArabidopsis Mo-sensitive mutants were selected. Subsequently, the phenotype, fresh weight and Mo content of mutant 2-4 were analyzed. The results showed that the growth of mutant 2-4 was sig- nificantly inhibited, and fresh weights and Mo contents of leaves and roots in mutant 2-4 were lower than those of wild type Col-0 on the -Mo medium. But fresh weights of leaves and roots of mutant 2-4 and wild type Col-0 were not obviously different on the ~Mo medium, and fresh weights and Mo contents were far higher than those on the -Mo medium.With the Mo content in the medium increasing, the symptoms of dwarf, leaf chlorosis and margin of leaf curl of mutant 2-4 recovered continuously. When the Mo content reached to 170 nmol.L1, the growth of mutant 2-4 had no obvious differences with the wild-type. These results showed that screened 2-4 olant was a Mo-sensitive mutant.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第2期171-177,共7页
Plant Physiology Journal
基金
教育部博士点专项科研基金(20123418120001)
安徽省高等学校自然科学研究项目(KJ2013A117)
关键词
拟南芥
钼
变异株
培养基
表型分析
Arabidopsis thaliana
molybdenum
mutant
medium
phenotypic analysis
