摘要
目的:观察电针十二指肠溃疡(DU)模型大鼠的小肠经经合穴"小海"、下合穴"下巨虚"对其血清中肿瘤坏死因子-α(TNF-α)及十二指肠组织中高迁移率族蛋白B 1(HMGB 1)表达的影响,初步探讨"合治内府"中"合"穴的相对特异性。方法:SD大鼠随机分为空白组、模型组、小海组、下巨虚组,每组10只。于大鼠右臀部皮下注射10%盐酸半胱胺建立DU模型,小海组电针"小海"穴,下巨虚组电针"下巨虚"穴,每次30min,每天1次,治疗10d。肉眼观察大鼠十二指肠溃疡情况并评分,双抗体夹心酶联免疫吸附法检测大鼠血清TNF-α含量,免疫组织化学法检测大鼠十二指肠组织中HMGB 1表达。结果:造模后大鼠十二指肠溃疡评分较空白组增高(P<0.01),小海组、下巨虚组的评分低于模型组(P<0.01),且下巨虚组低于小海组(P<0.01);模型组的血清TNF-α含量较空白组增高(P<0.01),小海组、下巨虚组的血清TNF-α低于模型组(P<0.01),且下巨虚组低于小海组(P<0.01);模型组十二指肠组织中HMGB 1表达高于空白组(P<0.01),下巨虚组十二指肠组织中的HMGB 1低于模型组(P<0.05)。结论:1电针小肠经经合穴、下合穴对十二指肠溃疡均可产生一定治疗作用,可能是通过下调血清TNF-α或十二指肠组织HMGB 1的表达来发挥抗炎作用的;且较之"小海"穴而言,"下巨虚"穴存在相对特异性。2本结果部分证实"合治内府"中的"合"主要应指下合穴。
Objective To observe the effect of electroacupuncture （EA） stimulation of ＂Xiaohai＂ （SI 8） and ＂Xiajuxu＂ （ST 39） on serum TNF-α and duodenal high mobility group protein B 1 （HMGB 1） levels in rats with duodenal ulcer （DU）, so as to analyze its underlying mechanism in improving DU. Methods Forty SD rats were randomly divided into normal control, model, Xiaohai （SI 8） and Xiajuxu （ST 39） groups （n = 10 in each group）. The DU model was established by intramuscular injection of 10% Cysteamine hydrochloride （300 mg/kg）, followed by taking water containing 1% Cysteamine hydrochloride for 48 h. EA （10 Hz/50 Hz, 1 -3 mA） was applied to bilateral SI 8 and ST 39 for 30 min, once a day for 10 days. The DU score （0--5 score） was evaluated according to Moraes; method. Serum TNF-a content was determined by ELISA and duodenal HMGB 1 expression was measured by immunohistochemistry. Results Compared to the normal control group, the DU score,serum TNF-a content, and duodenal HMGB 1 expression level of the model group were significantly higher （P〈0. 01 ）. Following EA intervention, the in- creased DU scOre,serum TNF-α content, and duodenal HMGB 1 expression level were considerably down-regulated in the two EAgroups in comparison with the model group （P〈0.01 ,P〈0.05） except HMGB 1 in the SI 8 group （P〉0.05）, and the effect of EA of ＂Xiajuxu＂ （ST 39）, the lower-He point, was remarkably superior to that of ＂Xiaohai＂ （SI 8） in reducing DU score and TNF-α content （P〈0.01）. Conclusion EA stimulation of SI 8 and ST 39 can improve duodenal ulceration in DU rats, which may be related to its effect in down-regulating serum TNF-α and duodenal HMGB 1 expression. The therapeutic effect of ST 39 is markedly better than SI 8 in relieving duodenal ulceration.
出处
《针刺研究》
CAS
CSCD
北大核心
2015年第1期35-39,共5页
Acupuncture Research
基金
国家自然科学基金项目(No.81173327)
