摘要
目的探讨野黄芩苷诱导人舌鳞癌Tca8113细胞凋亡的可能机制。方法将体外培养的人舌鳞癌Tca8113细胞分成对照组和不同浓度野黄芩苷组(浓度分别为80、120、160 mg/L),每组设3个复孔。采用Tunel检测细胞凋亡情况;采用免疫细胞化学法和Western blot法检测caspase-8蛋白表达情况。结果 (1)80、120、160mg/L野黄芩苷组Tca8113细胞凋亡率高于对照组[(17.63±0.25)%、(36.23±0.36)%、(51.84±0.58)%vs(4.45±0.27)%,P<0.05]。(2)与对照组相比,80、120、160 mg/L野黄芩苷组caspase-8蛋白的表达增加(0.283±0.040 vs0.474±0.031、0.592±0.077、0.781±0.020,P<0.05)。结论野黄芩苷能明显诱导舌鳞癌细胞Tca8113的凋亡,可能与上调caspase-8蛋白表达有关。
Objective To investigate the possible mechanism of scutellarin on the apoptosis of human tongue squa？mous carcinoma Tca8113 cells. Methods Human tongue squamous carcinoma Tca8113 cells were divided into control group and scutellarin groups （80, 120 and 160 mg/L）. Tunel method was used to detect the apoptosis. Immunohistochemistry and Western blot assay was used to detect the caspase-8 protein expression of cells. Results （1）The apoptotic rates of Tca8113 cells were significantly high in scutellarin groups （80, 120 and 160 mg/L） than those in control group [（17.63 ± 0.25）%, （36.23±0.36）%, （51.84±0.58）%vs （4.45±0.27）%, P〈0.05].（2）Compared with control group, the expressions of cas？pase-8 protein were significantly increased in different concentrations （80, 120 and 160 mg/L） of scutellarin groups （0.283± 0.040 vs 0.474±0.031, 0.592±0.077, 0.781±0.020,P〈0.05）. Conclusion Scutellarin could obviously induce the apoptosis of human tongue squamous carcinoma Tca8113 cells, which may be related to the caspase-8 protein expression.
出处
《天津医药》
CAS
2015年第3期237-240,共4页
Tianjin Medical Journal
