摘要
目的:建立同时分析测定芎菊上清片中4个活性成分(α-松油醇、薄荷酮、薄荷脑和胡薄荷酮)的方法。方法:采用GC法,色谱柱为DB-1701毛细管色谱柱(30 m×0.3 mm×0.25μm),柱温采用程序升温(80℃保持1 min,以5℃·min^(-1)的速率升温至120℃,保持5 min,再以50℃·min^(-1)的速率升温至220℃,保持2 min),进样口温度200℃,FID检测器,检测器温度230℃。结果:α-松油醇、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.01~0.55 mg·mL^(-1)(r=0.999 9)、0.10~0.50 mg·mL^(-1)(r=0.999 8)、0.01~0.50 mg·mL^(-1)(r=0.999 8)、0.01~0.51 mg·mL^(-1)(r=0.999 9);平均加样回收率(n=6)分别为97.6%、98.2%、95.7%、96.1%,其RSD分别为1.5%、1.4%、1.6%、1.6%。结论:该方法操作简单,重复性好,可有效控制芎菊上清片的质量。
Objective： To establish a method for simuhaneous determination of four active ingredients （ α- terpineol, menthone, menthol, pulegone） in Xiongjushangqing Tablets. Methods： GC method was adopted . The determination was performed on a DB-1701 column （30 m×0.3 mm ×0.25 μm）. The column temperature was programmed （maintained at 80 ℃ for 1 min, then risen to 120 ℃ at the rate of 5 ℃ · min^-1, held for5 min. After that the temperature was elevated to 220 ℃ at the rate of 50 ℃ · min^-1 and held for 2 min）. The FID was used as detector, the temperatures of the injection and the detector were set at 200 ℃ and 230 ℃. Results： The calibration curves were linear in the ranges of 0.01 -0.55 mg · mL^-1 for α- terpineol （r = 0.999 9） , 0. 10 -0.50 mg · mL^-1 for menthone（r = 0. 999 8） , 0.01 -0.50 mg · mL^-1 for menthol （r =0.999 8） and 0.01-0.51 mg· mL^-1 for pulegone （r=0.999 9）. The average recoveries （n=6） were 97.6%,98.2%,95.7% and 96. 1 %. RSD were 1.5% , 1.4% , 1.6% and 1.6% , respectively. Conclusion： The method is simple and repeatable, which can be used for quality control of Xiongjushangqing Tablets.
出处
《中国药品标准》
CAS
2015年第1期8-10,共3页
Drug Standards of China
