摘要
目的 检测过氧化物还原酶3（peroxiredoxin 3,PRX3）在宫颈上皮转染人乳头瘤病毒（human papillomavirus,HPV） 16型之后的表达变化,以探讨PRX3在宫颈癌发生中的作用.方法 切取正常宫颈转化区的上皮组织进行贴壁培养,用含有HPV16 E6/E7基因的重组pcDNA3.1载体转染宫颈上皮细胞.待建立稳定表达HPV16 E6/E7的细胞系以后,通过实时定量PCR和蛋白质印迹法检测转染前后PRX3表达水平的变化.结果 在稳定表达HPV16 E6/E7基因的宫颈上皮细胞中,PRX3的表达水平（包括mRNA和蛋白）显著降低.结论 高危型HPV16与宫颈上皮细胞发生整合之后,通过诱导包括PRX3在内的一系列基因的表达变化,导致宫颈上皮的恶性转化及宫颈癌的发生.
Objective We previously demonstrated that peroxiredoxin 3 （PRX3） was overexpressed in cervical cancer and the expression of PRX3 was positively associated to that of oncogenes E6/E7 of human papillomaviruses （HPV） type 16.The present study was conducted to investigate the link between HPV oncogenes and PRX3,which would be helpful to understanding the mechanism for cervical carcinogenesis.Methods Cervical epithelial cells were cultured and were transfected with recombinant pcDNA3.1 vector containing HPV16 E6/E7.The changes of PRX3 expression were detected by quantitative real time PCR and Western blotting.Results In the epithelial cells which stably expressed HPV16 E6/E7,the expression of PRX3 was significantly down-regulated at both mRNA and protein levels compared to that in the control cells.Conclusion Integration of high risk HPV DNA into the chromosomes of cervical epitheliums induced the change of the PRX3 expression,which might be involved in the initiation of cervical cancer.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第1期38-40,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金资助（项目编号30872938）
