摘要
目的研究蛋白激酶CK2β及抑癌基因蛋白p53在卵巢癌组织中的表达情况,并分析其表达与卵巢癌临床病理参数及预后的关系。方法收集2003-01-01-2005-12-31就诊于浙江大学医学院附属妇产科医院的上皮性卵巢肿瘤患者148例(包括108例上皮性卵巢癌,20例卵巢良性上皮性肿瘤及20例卵巢交界性肿瘤),应用免疫组化法检测CK2β及p53在肿瘤组织中的表达情况,并分析两者在不同肿瘤组织中的表达差异及其与上皮性卵巢癌预后的关系。结果上皮性卵巢癌组织CK2β阳性表达68例(63.0%),p53阳性表达58例(53.7%);卵巢交界性肿瘤CK2β阳性表达6例(30.0%),p53阳性表达4例(20.0%);卵巢良性上皮性肿瘤CK2β和p53阳性表达均为0例;CK2β在卵巢癌组织中的表达强度显著高于卵巢良性肿瘤组织(P<0.001)和交界性肿瘤组织(P=0.003);p53在卵巢癌组织中的表达强度显著高于卵巢良性肿瘤组织(P<0.001)及交界性肿瘤组织(P=0.006);CK2β和p53之间具有显著相关性,r=0.377,P<0.001。CK2β蛋白表达与卵巢癌临床分期(P=0.001)、术前血清CA125水平(P<0.001)及化疗敏感性(P=0.012)显著相关;p53蛋白表达与组织分化程度(P=0.027)及术前血清CA125水平(P=0.027)相关。CK2β及p53阳性表达与上皮性卵巢癌患者的无瘤生存预后及总生存预后显著相关(P值均<0.05);多因素回归显示,临床分期(P<0.001)和p53阳性表达(P=0.018)可作为预测卵巢癌无瘤生存预后的独立因素,而临床分期(P=0.001)、术前CA125水平(P=0.040)和p53阳性表达(P=0.041)可作为预测卵巢癌总生存预后的独立因素。结论 CK2β和p53蛋白阳性表达可能与卵巢癌的发生发展有密切关系,p53阳性表达对卵巢癌预后预测具有一定的价值。
OBJECTIVE To investigate the expressions of CK2βand p53 in epithelial ovarian cancer tissues and to analysis their correlation with clinical pathological and survival significance. METHODS 108 patients with epithelial o- varian cancer,20 patients with benign epithelial ovarian tumor and 20 patients with bordline epithelial ovarian tumor who underwent treatment in Women＇s Hospital,School of Medicine,Zhejiang University between January 1,2003 and Decem- ber 31,2005 were retrospectively analyzed. Immunohistoehemistry was used to assess the expressions of CK2β and p53. The differences among different epithelial ovarian tumor and the relationships between expression of CK2β, p53 and prog- nosis in epithelial ovarian cancer were analyzed. RESULTS The positive expression rate of CK2β and p53 in epithelial o- varian cancer, bordline ovarian tumor and benign ovarian tumor was 63.0% （68/108） and 53.70% （58/108）, 30.0% （5/20） and 20. 0% （4/20）,0 （0/20） and 0 （0/20） respectively. The expression intensities of CK2β and p53 in epithelial ovarian cancer were signifieanly higher than that in benign（P〈0. 001, P〈0. 001）and bordline epithelial ovarian tumor tissues （P = 0. 003, P = 0. 006）, and there was significant correlation between CK29 and p53 expression （ r ~ 0. 377, P〈 0. 001 ）. The expression of CK2β protein in epithelial ovarian cancer was associated with tumor stage（P = 0. 001）, CA125 level （P^0. 001） and chemosensitivity（P =0. 012） whereas the expression of p53 protein in epithelial ovarian cancer was correlated with histological grade （P=0. 027） and CA125 level（P= 0. 027）. Patients with positive expression of CK213 and p53 demonstrated a significantly worse prognosis than that of patients with negative expression of CK213 and p53 for disease-free and overall survival（all P〈0.05）. Multivariate survival analysis revealed that tumor stage （P〈0. 001） and positive p53 expression （P = 0.018） provided significant independent prognostic value for disease-free survival, whereas tumor stage （P = 0.001 ）, CA125 level （P= 0. 040） and p53 expression （P = 0. 041 ） provided significant independent prognostic value for overall survival. CON- CLUSIONS The expressions of CK2β and p53 protein were significantly associated with the development and progression of epi- thelial ovarian cancer, p53 expression has important predictive value in predicting ovarian cancer survival.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2015年第6期451-456,共6页
Chinese Journal of Cancer Prevention and Treatment
基金
国家高技术研究发展计划(863计划
2012AA02A507)
