摘要
目的研究多梳基因Bmi-1在甲状腺乳头状癌组织中的表达及临床意义。方法分别采用免疫组化法、Western blot法和RT-PCR法检测30例甲状腺乳头状癌组织、30例结节性甲状腺肿组织及30例正常甲状腺组织中多梳基因Bmi-1及其mRNA的表达情况,并分析Bmi-1与甲状腺乳头状癌临床病理特征的关系。结果免疫组化法检测发现Bmi-1在甲状腺乳头状癌组织中阳性表达率73.3%,与结节性甲状腺肿(36.7%)及与正常组织(16.7%)的阳性表达率相比差异有统计学意义(P<0.05)。Western blot法检测甲状腺乳头状癌中Bmi-1表达量与结节性甲状腺肿组织及正常组织相比,差异有统计学意义(P<0.05)。RT-PCR法检测甲状腺乳头状癌中Bmi-1 mRNA表达量与结节性甲状腺肿组织及正常组织相比,差异有统计学意义(P<0.05)。Bmi-1阳性表达在甲状腺乳头状癌有无淋巴结转移中的差异有统计学意义(P<0.05),在年龄、性别、肿瘤大小、分期等差异无统计学意义。结论 Bmi-1在甲状腺乳头状癌中表达升高,可能在甲状腺乳头状癌的发生、发展过程中起重要作用,并可望作为甲状腺乳头状癌临床诊断的标记物之一。
Objective To investigate the expression and clinical significance of Bmi-1 in papillary thyroid carcinoma. Methods The expression of Bmi-1 mRNA and protein was detected by RT-PCR, Western blot and immunohistochemistry in 30 samples of papillary thyroid carcinomas, 30 samples of nodular goiters and 30 samples of normal thyroid tissue, respectively. The correlation of Bmi-1 expression with ciinicopathological features of patients was also analyzed. Results The results of immunohistochemistry showed that the positive rates of Bmi-1 protein in papillary thyroid carcinomas,nodular goiters, normal thyroid tissues were 73.3%, 36.7% and 16.7%, respectively（P〈0.05）. Western blot showed that the expression of Bmi-1 protein in papillary thyroid carcinomas was significantly higher than those in nodular goiter tissue and normal tissue（P〈 0.05）. The expression of Bmi-lmRNA in papillary thyroid carcinomas was significantly higher than those in nodular goiters and normal thyroid tissues （P〈0.05）. Bmi-1 expression was closely relevant to lymph node metastasis （P〈0.05）, but not to gender, age and differentiation degree. Conclusion The expression of Bmi-1 gene is up-regulated in papillary thyroid carcinomas, indicating that Bmi-1 may be involved in the development of papirlary thyroid carcinoma and may serve as a marker for clinical diagnosis.
出处
《浙江医学》
CAS
2015年第4期287-290,310,共5页
Zhejiang Medical Journal
基金
杭州市医药卫生科技计划项目(2012A008)
杭州市重大科技创新专项项目(20131813A08)
