摘要
目的建立桔梗流浸膏的指纹图谱检测方法,以达到质量的均一和稳定。方法采用UPLC-ELSD法,乙腈-水梯度洗脱,色谱柱:ACQUITY UPLC BEH C18(2.1mm×100mm,1.7μm),柱温:30℃,流速:0.2ml/min,在20min内,得到11个分离度良好的色谱峰。结果采用中药色谱指纹图谱相似度软件计算,桔梗流浸膏样品相似度均大于0.90,符合指纹图谱要求。结论该方法经稳定性、重复性、精密度考察,是一种快速、准确的中药质量评价方法。
Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its u‐niformity and stability .Methods UPLC‐ELSD was applied to obtain a chromatogram with 11 well separated peaks ,and the chromatographic condition was as follows：mobile phase was gradient elution by acetonitrile‐water ,column was ACQUITY UPLC BEH C18 （2 .1 × 100 mm ,1 .7 μm） with the column temperature of 30 ℃ and the flow rate of 0 .2 ml/min .Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM .Similar‐ities were all greater than 0 .90 ,which could meet the requirement for chromatographic fingerprint of TCM .Conclusion The stability ,repeatability and precision of the developed method were all validated ,and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine .
出处
《药学实践杂志》
CAS
2015年第2期153-155,共3页
Journal of Pharmaceutical Practice
基金
国家药品标准提高暨2015版药典科研项目(TSP021)
