摘要
目的比较3种消毒方法对变形链球菌(S.mutans)在人牙本质片上黏附的影响,为牙体标本消毒提供参考。方法制备人牙本质片60个,随机均分为3组,每组20个,采用不同的方法消毒。第1组:高压蒸汽灭菌30 min;第2组:10%福尔马林溶液浸泡1周;第3组:3%过氧乙酸溶液浸泡1周。将不同消毒方法的牙本质片与S.mutans进行体外黏附实验,采用扫描电子显微镜(SEM)观察牙本质片表面形态变化及S.mutans黏附量多少,并采用菌落形成单位计数法(CFU)计数S.mutans在不同消毒方法的牙本质表面的黏附量,用SPSS 13.0软件对实验结果进行统计学分析。结果不同消毒方法的牙本质片在电子显微镜下表面形态无明显变化,SEM显示S.mutans在三组牙本质片上黏附变化比较,差异无统计学意义(F=3.21,P>0.05),CFU显示S.mutans培养后3组黏附差异无统计学意义(F=3.29,P>0.05)。结论 3种消毒方法对体外研究细菌黏附于牙本质片影响甚微,目前高压蒸汽灭菌法简单、可靠、易于操作,应作为消毒方法的首选。
Objective To compare the influence of 3 different sterilization methods on adherence of S.mutans to dentin slices. Methods 60 dentin slices were randomly divided into three groups with 20 specimens in each group, they were given 3 different sterilization methods. The first group of detin slices was disinfected by autoclaving for 30 min; the second group was immersed in 10% formalin solution for 1 week; and the third group was immersed in 3% peracetic acid for 1 week. The quantification of attached S.mutans on the surfaces of different dentin was assayed by means of scanning electron microscope（SEM） and clone forming unit（CFU） method in vitro. Results There was no obvious change in morphology among the three groups of dentin slices under the electron microscope. The quantification of attached S.mutans was no statistically significant difference among the three groups under SEM（F = 3.21, P〉0.05）, and there was no statistically significant difference from CFU after bacterial culturer（F = 3.29, P〉0.05）. Conclusion Little effect is observed in the test of bacterial adhesion to dentin slices of different sterilization methods. So far, autoclaving is seen as the simple and reliable sterilization method.
出处
《中国医药导报》
CAS
2015年第9期114-117,共4页
China Medical Herald
