摘要
以玉米小斑病菌C小种毒素(HMC-toxin)分别诱导2对同核异质体玉米的幼苗根冠细胞(即:基因型为B37的玉米C型不育细胞质CB37和同核保持系NB37,基因型为Mo17的玉米C型不育细胞质CMo17和同核保持系NMo17),采用吖啶橙(AO)与溴化乙啶(EB)荧光复染的方法,研究细胞发生凋亡的差异性及凋亡规律。结果表明:CB37的幼叶接种PBS再经150mg/L的HMC毒素处理后,其根冠细胞凋亡率达最高值为45.3%,而NB37仅为15.3%;CMo17凋亡率最高达63.7%,NMo17仅为6.39%;C细胞质的细胞死亡率远高于N细胞质,凋亡率随诱导浓度和诱导时间的增加而增加。HMC毒素诱导根冠细胞发生凋亡,活细胞核发出绿色荧光,坏死细胞核则呈现橙红色荧光,且细胞核呈松散的颗粒状或新月状,凋亡细胞则发出黄绿色或绿色荧光,染色体固缩成新月状或颗粒状。
To study the feature of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from two pair of homokaryon C and N inbred line of maize. Acridine orange （AO） and ethidium bromide （EB） staining methods were used to detach the apoptosis of root cap cells. In the process of induction, root cap cells appeared apoptotic bodies and marginate of chromosome morphology. Living, necrosis and apoptosis cells could be distinguished clearly by AO and EB complex staining. The result showed that the ratio of apoptosis in C cells was sharply higher than thatof N, and the max ratios of apoptosis of B37-C and B37-N stained by AO and EB were 45.3% and 15.3%, respectively. In contrast, the max ratios of apoptosis of Mol7-C and Mol7~N stained by AO and EB were 63.7% and 6. 39%, respectively. It is concluded that harmed by HMC toxin, the root cap cells appear apoptosis. The ratio of apoptosis in C cytoplasm was higher than in N cytoplasm. Along with the increase of toxin concentration and treating time, the ratio of apoptosis became higher HMC toxin induced root cap cells apoptosis, cell nucleus issued a green fluorescent, necrotic nuclei are orange red fluorescence, and loose granular cell nucleus or crescent, apoptotic cells emit yellow or green fluorescence, chromosome becomes pyknotic crescent or granule shape.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第2期192-197,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
科技部科技伙伴计划(KY201402017)
河北省自然科学基金(C2011301010)
河北省高层次人才项目(A2010003028)
河北省科技计划项目(13396401D)
农业部"948"[2011-G1(4)-07]
关键词
玉米小斑病菌C小种
毒素
细胞凋亡
吖啶橙
溴化乙啶
Bipolaris maydis race C （HMC）
toxin
apoptosis
acridine orange （AO）
ethidium bro-mide （EB）
