摘要
目的：检测活性氧调节因子1（ reactive oxygen species modulator 1，Romo1）在胃癌组织及外周血中的表达，分析其与胃癌临床病理特征的相关性及其意义。方法采用免疫组化S－P法检测40例胃癌患者癌组织及相应的癌旁组织（距癌组织边缘＞5 cm）中Romo1蛋白表达；同时用酶联免疫吸附（ ELISA）法检测40例胃癌患者及40例健康对照者外周血中Romo1蛋白浓度。结果免疫组化结果显示，胃癌组织中Romo1蛋白阳性表达率（72．5％）显著高于癌旁组织（17．5％）（P＜0．01）。胃癌患者外周血中Romo1蛋白浓度为（289．0±51．3）ng／L，显著高于对照组（75．2±18．9）ng／L（P＜0．01）。癌组织中Romo1蛋白表达及外周血中Romo1蛋白浓度均与肿瘤浸润深度、TNM分期、淋巴结转移相关（P＜0．05或P＜0．01），胃癌患者癌组织与外周血中Romo1蛋白表达呈正相关（r＝0．774，P＜0．01）。结论 Romo1在胃癌患者癌组织及外周血中高表达，其表达与肿瘤浸润深度、TNM分期、淋巴结转移有关，Romo1可能在胃癌的发生过程中起到重要作用；外周血中Romo1浓度能够可靠地反映癌组织中Romo1蛋白表达情况，可作为一种用于胃癌诊断、病情监测的新肿瘤标志物。
Objective To examine the amounts of reactive oxygen species modulator 1 （ Romo1） in the neoplastic tissues and peripheral blood of gastric cancer patients , and to analyze its relationship with the pathological characteristics of gastric cancer .Methods The levels of Romo1 were determined using the neoplastic tissues and corresponding adja-cent normal tissues （〉5 cm from cancer edge ） collected from 40 gastric cancer patients through immunohistochemistry . Meanwhile, the amounts of Romo1 within the peripheral blood of 40 gastric cancer patients and 40 healthy subjects were measured using enzyme -linked immunosorbent assay （ ELISA ） .Results According to immunohistochemistrical re-sults, gastric cancer tissues showed a significant increase in the positive rate of Romol （72.5%） than adjacent normal ones （17.5%） （P〈0.01）.The level of Romo1 was measured as （289.0 ±51.3） ng/L in the peripheral blood of gas-tric cancer patients, which were remarkably higher than that of healthy subjects （75.2 ±18.9） ng/L （P〈0.01）.The expressions of Romo1 in the neoplastic tissues and peripheral blood were correlated with the depth of infiltration , TNM stage, and lymph node metastasis （P〈0.05 or P〈0.01）.The amount of Romo1 in the neoplastic tissues was positively correlated with that in the peripheral blood （r=0.774, P〈0.01）.Conclusion The levels of Romo1 were enhanced in the neoplastic tissues and peripheral blood of gastric cancer patients , which may be related with the depth of infiltra-tion, TNM stage and lymph node metastasis .The concentration of Romo 1 within the peripheral blood can reflect the ex-pression of this protein within the neoplastic tissues , which indicates the role of Romol as a new tumor marker for better diagnosing and monitoring of gastric cacner .
出处
《徐州医学院学报》
CAS
2015年第2期90-93,共4页
Acta Academiae Medicinae Xuzhou
关键词
胃癌
活性氧调节因子1
gastric cancer
reactive oxygen species modulator 1
