摘要
目的探讨联合检测辅助性T淋巴细胞(Th)1、2型细胞因子的表达在急性胰腺炎(AP)的病情严重程度及疗效评估中的价值。方法 2009年11月至2013年6月,采用ELISA法分别测定32例轻型胰腺炎(MAP组)与18例重症胰腺炎(SAP组)患者住院第1、4、7天和15例健康者(健康对照组)的Thl型细胞因子干扰素-γ(IFN-γ)和Th2型细胞因子白细胞介素-4(IL-4)表达水平。结果 MAP组住院第1、4、7天IFN-γ的检测结果分别为(31.47±4.35)、(36.92±4.12)、(49.09±4.97)ng/L;IL-4的表达水平分别为(54.72±5.52)、(45.26±4.89)、(39.79±3.85)ng/L。SAP组住院第1、4、7天的IFN-γ表达水平分别为(23.97±5.39)、(26.29±3.47)、(33.64±4.47)ng/L;IL-4的表达水平分别为(70.45±5.05)、(65.99±4.58)、(56.23±4.23)ng/L,健康对照组IFN-γ和IL-4的检测结果分别为(49.26±20.67)、(35.15±16.32)ng/L。与健康对照组相比,MAP组除第7天外的Th1型细胞因子IFN-γ和SAP组外周血Th1型细胞因子IFN-γ表达降低,Th2型细胞因子IL-4表达升高,差异有统计学意义(P<0.05);在相同治疗天数,SAP组各检测值与MAP组比较差异有统计学意义(P<0.05)。结论 Th1型细胞因子和Th2型细胞因子在AP存在明显极化,其极化程度与病情严重程度有关,联合检测Th1型细胞因子和Th2型细胞因子有利于判断AP的病情严重程度和治疗效果。
Objective To explore the value of the combined detection of helper T lymphocyte （Th）1 and 2 type cytokines ex‐pression in the evaluation of acute pancreatitis severity and treatment efficacy .Methods From May 2009 to June 2013 ,the enzyme‐linked immunosorbent assay （ELISA ） was adopted to detect the expression levels of Th1‐type cytokineinterferon‐γ （IFN‐γ） and Th2‐type cytokine interleukin （IL）‐4 in 32 cases of mild acute pancreatitis （MAP group） and 18 cases of severe acute pancreatitis （SAP group） on 1 ,4 ,7 d after admission as well as 15 cases of normal healthy people （control group） .Results The test results of IFN‐γ on 1 ,4 ,7 d after admission in the MAP group were （31 .47 ± 4 .35） ,（36 .92 ± 4 .12） ,（49 .09 ±4 .97）ng/L ,which of IL‐4 were （54 .72 ± 5 .52） ,（45 .26 ± 4 .89） ,（39 .79 ± 3 .85）ng/L .The test results of IFN‐γ on 1 ,4 ,7 d after admission in the SAP group were （23 .97 ± 5 .39） ,（26 .29 ± 3 .47） ,（33 .64 ± 4 .47）ng/L ,and which of IL‐4 were （70 .45 ± 5 .05） ,（65 .99 ± 4 .58） ,（56 .23 ± 4 .23）ng/L .The test results of IFN‐γ and IL‐4 in the normal control group were （49 .26 ± 20 .67）ng/L and （35 .15 ± 16 .32）ng /L respectively .Compared with the normal control group ,the expressions of Th1‐type cytokine IFN‐γ in the MAP group （except which on 7 d） and peripheral blood Th1‐type cytokine IFN‐γ in the SAP group were decreased and the expression of Th2‐type cyto‐kine IL‐4 was increased ,the difference was statistically significant （P〈0 .05） ;The test results of each index in the same treatment time had statistically significant differences between the SAP group and the MAP group （P〈0 .05） .Conclusion The obvious po‐larization of Th1‐type cytokine and Th2‐type cytokine exists in acute pancreatitis ,and the polarization degree is related with the se‐verity of disease .The combined detection of Th1‐type cytokine and Th2‐type cytokine is beneficial to judge the extent of serious condition and treatment effect of acute pancreatitis .
出处
《国际检验医学杂志》
CAS
2015年第6期749-750,752,共3页
International Journal of Laboratory Medicine
