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桔全爪螨乙酰胆碱酯酶(AChE)生化特性研究 被引量:1

Biochemical Characteristics of Acetylcholinesterase(AChE) in the Citrus Red Mite,Panonychus citri McGregor(Acari: Tetranychidae)
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摘要 为明确桔全爪螨乙酰胆碱酯酶AChE与其抗药性的关系,应用微量滴度酶标板法对桔全爪螨敏感品系(SS)、甲氰菊酯抗性品系(FeR)和阿维菌素抗性品系(AvR)的乙酰胆碱酯酶(AChE)的生化特性进行了比较研究.结果表明:3个品系中,以甲氰菊酯抗性品系FeR的AChE活力、比活力以及最大反应速率Vmax值最大;两抗性品系的AChE活力、比活力和Vmax值均显著高于敏感品系(P<0.05),且两抗性品系之间也存在显著差异(P<0.05);米氏常数Km值在3个品系间无显著差异(P>0.05).这说明AChE活力提高是桔全爪螨对甲氰菊酯和阿维菌素产生抗性的原因之一. In order to clarify the relationship between AChE and resistance of Panonychus citri, the biochemical characteristics of AChE from three strains ( Susceptible strain, SS ; Fenpropathrin resistance strain, FeR ; Avermectin resistance strain,AvR) of P. cirri were studied by microplate reader method. The results showed that, among three strains, the activity per mite and specific activity of AChE,as well as the V value were highest in FeR. Compared to SS strain,these values in FeR and AvR were significantly higher (P 〈 0.05) , and significant difference was ob- served between two resistant strains (P 〈 0.05). However, there was no significant difference of K value among three strains (P 〉 0.05). The above results indicated that increased activity of AChE can play important roles in fenpropathrin and avermectin resistance in P. citri.
出处 《西华师范大学学报(自然科学版)》 2015年第1期12-16,共5页 Journal of China West Normal University(Natural Sciences)
基金 公益性行业(农业)科研专项经费项目(201103020) 四川省教育厅项目(11ZA293) 西华师范大学博士启动基金(10B029)
关键词 桔全爪螨 乙酰胆碱酯酶(AChE) 甲氰菊酯 阿维菌素 抗性 Panonychus citri AChE fenpropathrin avermectin resistance
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  • 1冉春,陈洋,袁明龙,王进军,刘浩强,姚廷山.桔全爪螨田间种群对杀螨剂的敏感性[J].植物保护学报,2008,35(6):537-540. 被引量:22
  • 2陈洋,冉春,熊琳,王进军.不同柑桔种质资源对桔全爪螨酶活性及其对药剂敏感性的影响[J].西南农业学报,2006,19(3):434-437. 被引量:8
  • 3冉春,陈志永,陈洋,王进军.桔全爪螨田间种群对双甲脒的敏感性及其两种酶的生化特性[J].中国农学通报,2007,23(11):284-288. 被引量:5
  • 4RAN C,CHEN Y,WANG J J.Susceptibility and Carboxylesterase Activity of Five Field Populations of Panonychus citri (McGregor)(Acari:Tetranychidae) to Four Acaricides[J].International Journal of Acarology,2009,35:115-12.
  • 5刘永华,蒋红波,袁明龙,樊钰虎,杨丽红,陈静,王进军.柑橘全爪螨对4种杀螨剂的抗性监测及增效作用[J].果树学报,2010,27(4):570-574. 被引量:39
  • 6唐振华.昆虫抗药性[M].北京:中国农业出版社,1993,302-335.
  • 7FAO.Tentative Method for Spider Mites and Their Eggs,Tetranychus spp.and Panonychus ulmi (Koch)[J].FAO Plant Protection Bulletin,1974,22:103-107.
  • 8BRADFORD M M.A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Using the Principle of Proteindye Release[J].Analytical Biochemistry,1976,72:248-254.
  • 9REN Y,WEI X P,WU S,et al.Comparison of Acetylcholinesterase from Three Field Populations of Liposcelis paeta Pearman (Psocoptera:Liposcelididae):Implications of Insecticide Resistance[J].Pesticide Biochemistry and Physiology,2008,90:196-202.
  • 10ELLMAN G L,COURTNEY K D.A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity[J].Biochemical Pharmacology,1961,7:88-90.

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