摘要
利用菜豆根瘤菌Rhizobium etli CFN42全基因组测序结果,结合计算机技术和生物信息学分析方法,对菜豆根瘤菌蛋白质组中的分泌蛋白进行预测和分析,以便更深入地了解共生固氮的分子机制。经过分析发现,在该根瘤菌5 963个蛋白质中,具有信号肽的分泌蛋白332个,占总数的5.4%主要涉及底物转运、物质代谢和信号转导等方面。在有功能描述的186个分泌蛋白中,识别了Ⅰ、Ⅱ、Ⅲ、Ⅳ型分泌系统和丝氨酸蛋白酶,这些蛋白质可能参与了根瘤菌对宿主植物的侵染,是两者分子对话的关键参与者;在假定蛋白中,通过结构域分析,发现了SH3b、BA14K和EFh结构域,含有这些结构域的分泌蛋白可能参与了根瘤信号转导途径。在根瘤菌共生结瘤过程中,分泌蛋白发挥了重要作用。
In order to understand the molecular mechanism of the symbiotic nitrogen fixation better, in the pre- sent study, we predicted and analyzed the secreted proteins of Rhizobium etli CFN42, based on the available genome sequence of Rhizobium etli CFN42, by computer technology and bioinformatic methods. Our results showed that 332 of the 5 963 proteins in CFN42 were secreted proteins, accounting for 5.4% of the total number, mainly involving in substrate transport, material metabolism, and signal transduction etc. We identified type Ⅰ , Ⅱ,Ⅲ, Ⅳ secretion systems and serine proteases within which 186 proteins were functional annotated, which may involve in the host plant infection process and are key participants of the molecular dialogue, We also found SH3b, BA14K and EFh domains by SMART software within the hypothetical proteins, these domain-containing secreted proteins may participate in the root nodule signal transduction pathway. Proteins secreted by some rhizobias play an important role in establishment of a mutually beneficial symbiosis.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2014年第5期961-969,共9页
Genomics and Applied Biology
基金
大理学院青年教师科研基金项目(KYQN201217)
云南省高校滇西生物多样性科技创新团队基金资助项目(云教函〔2011〕93号)
大理学院质量工程项目(TDⅠ-05)共同资助
关键词
菜豆根瘤菌
全基因组
分泌蛋白
信号肽
Rhizobium etli, Whole genome, Secreted protein, Signal peptides
