摘要
板栗疫病菌(Cryphonectria parasitica)是引起板栗疫病的病原真菌。低毒病毒是一类侵染板栗疫病菌的无衣壳正链RNA病毒。无病毒栗疫病菌野生型菌株在PDA培养基上形成桔黄色菌落,而带毒株在则形成白色菌落。本研究利用已知的不同机制的抗病毒药物处理无病毒和感染病毒的板栗疫病菌菌株,观察菌丝体颜色变化并检测菌丝体中病毒双链RNA(dsRNA)的含量。结果显示,抗病毒药物处理后,带毒株系EP721和Euro7的菌丝体颜色表型发生明显变化,且病毒dsRNA累积量与菌丝体颜色变化存在明显相关性:随着药物浓度增大,菌丝体颜色加深,病毒dsRNA积累量下降。因此,可以根据菌株颜色的变化判断药物对病毒复制或症状表现是否有效,从而极大简化抗RNA病毒药物的初步筛选过程。
Cryphonectria parasitica is the causative agent for the chestnut blight. Hypoviruses are a group ofunen- capsidated, plus-sense RNA viruses that infect C. parasitica and render the fungus to become hypovirulent. The colony of the fungus is orange when grown on the PDA medium but white once it is infected by a hypovirus. Vari- ous antiviral drugs were supplemented into the media on which virus-free or virus-containing C. parasitiea strains were cultured to evaluate the efficacy of the drugs on the change of colony pigmentation and the accumulation of viral double-stranded RNA （dsRNA）. The results revealed that pigmentation of virus-infected hyphae and accumu- lation of CHVI-EP713 dsRNA was sensitive in response to some antiviral drugs. Thus, the change in pigment of fungal hyphae can be used as an indicator for effectiveness of antiviral drugs, greatly simplifying the initial screen- ing process for antiviral compounds.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2014年第5期975-981,共7页
Genomics and Applied Biology
基金
国家自然科学基金(31260027)
国家863计划(2001AA223111
2004AA223100)共同资助
关键词
药物筛选模型
板栗疫病菌
低毒病毒
Drug screening model, Cryphonectria parasitica, Hypovirus
