摘要
目的:观察氧化苦参碱(oxymatrine,OM)对脂多糖(lipopolysaccharide,LPS)诱导胰腺星状细胞(LTC-14细胞株)中核因子-κB(nuclear factorκB,NF-κB)表达和核易位的影响.方法:体外培养LTC-14细胞,分别用相应浓度的LPS和/或OM刺激后,检测NF-κB的表达.用MTT法检测细胞增殖活性,免疫细胞化学技术检测LTC-14细胞胞浆和胞核内NF-κB的表达,实时荧光定量PCR检测细胞内NF-κB mRNA的表达,Western blot法检测NF-κB蛋白含量.结果:OM呈时间-剂量依赖性地抑制LTC-14细胞增殖,LPS(10μg/mL)刺激LTC-14细胞引起NF-κB mRNA及其蛋白表达量增高,NF-κB核内易位量明显增加,OM干预后可以下调NF-κB mRNA和蛋白表达,抑制NF-κB核内易位.结论:对LPS诱导的LTC-14细胞NF-κB mRNA、蛋白表达及NF-κB向核内易位抑制作用可能是OM治疗胰腺纤维化的机制之一.
AIM：To investigate the effect of oxymatrine（OM） on lipopolysaccharide（LPS） induced expression of nuclear factor kB（NF-kB） in pancreatic stellate cells（LTC-14）.METHODS：LTC-14 cells were cultured with suitable concentrations of LPS as well as the corresponding concentrations of OM.Cell proliferation was detected by MTT assay.The expression of cytoplasmic and nuclear NF-kB was detected by immunohistochemistry.The expression of NF-κB mRNA was evaluated by Q-PCR.The protein expression of NF-kB was measured by Western blot.RESULTS：OM inhibited the proliferation of LTC-14 cells in a time- and dose-dependent manner.The expression of NF-κB mRNA and protein was significantly increased in LTC-14 cells incubated with LPS（10 μg/mL）,which could be down-regulated by OM.The nuclear translocation of NF-kB could also be inhibited by OM.CONCLUSION：The reduction of NF-κB mRNA and protein as well as the inhibition of NF-kB nuclear translocation might be involved in the therapeutic effects of OM on pancreatic fibrosis.
出处
《世界华人消化杂志》
CAS
2015年第5期761-766,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81173393
武警后勤学院创新团队基金资助项目
No.WHTD201310~~
