摘要
目的:观察磁性四氧化三铁纳米粒子(Fe3O4)在交变磁场(EMF)作用下对肝癌细胞凋亡相关因子的影响。方法:培养大鼠肝癌CBRH-7919细胞,分为正常对照组、交变磁场照射组、100μg/m L Fe3O4纳米粒子组、交变磁场+100μg/m L纳米Fe3O4组。酶生化法检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;Western Blot检测Bax、Bcl-2表达及细胞色素C从线粒体至胞浆的释放;荧光酶标仪测定Caspase-3活性。结果:交变磁场协同磁性Fe3O4纳米粒子作用于CBRH-7919细胞能使Bax表达上调,而凋亡抑制基因Bcl-2表达下调,促进细胞色素C从线粒体释放至胞浆,增加Caspase-3活性。交变磁场、磁性Fe3O4纳米粒子单独作用组对结果无影响。结论:在交变磁场作用下,Fe3O4纳米粒子诱导CBRH-7919细胞凋亡的机制与Bax表达增加、Bcl-2表达减少、细胞色素C的释放及Caspase-3活性增加有关。
Objective To observe the influence of magnetic Fe304 nanoparticles on the apoptosis-related factors in hepatoma car- cinoma cells under the effect of alternating magnetic field. Methods The hepatoma carcinoma cells CBRH-7919 of rats were cultivated and divided into the normal control group, the alternating magnetic field exposure group, 100 ixg/mL nano Fe304 group, and the alternating magnetic field ＋ 100 p,g/mL nano Fe304 group. Superoxide dismutase（SOD） activity and malondialde- hyde （MDA） content were measured by biochemical methods; the expression of Bax and Bel-2 and the release of cytochrome C from mitochondria to the cytoplasm were investigated by western blotting. Caspase-3 activity was detected by fluorescence mi- croplate reader. Results The synergistic effect of alternating magnetic field and magnetic Fe304 nanoparticles on CBRH-7919 cells can make Bax expression up-regulated and Bcl-2 expression down-regulated. Meanwhile, it can promote the release ofcy- tochrome C from mitochondria to the cytoplasm and increase the caspase-3 activeness. The alternating magnetic field or magnet- ic Fe304 nanoparticles had no extra effect on the results alone. Conclusion Under the effect of alternating magnetic field, the mechanism of magnetic Fe304 nanoparticles inducing apoptosis in CBRH-7919 cells is connected with the increase of Bax ex- pression, the reduction of Bcl-2 expression, the release ofcytochrome C and the increase ofcaspase-3 activeness.
出处
《中国医学物理学杂志》
CSCD
2015年第2期255-258,共4页
Chinese Journal of Medical Physics
基金
滨州医学院科技计划项目(BY2010KJ084)
关键词
四氧化三铁
纳米粒子
交变磁场
肝癌细胞
凋亡
Fe304
nanoparticles
alternating magnetic field
hepatoma carcinoma cells
apoptosis
