摘要
以青葙茎段为外植体建立高频再生体系,探讨不同激素配比对青葙不定芽诱导、继代增殖和不定根诱导的影响。结果表明：青葙茎段外植体诱导培养基为MS＋6-BA（2mg/L）＋IBA（0.5mg/L）,诱导率为100%;继代增殖培养基为MS＋6-BA（2mg/L）＋IBA（0.01∽0.02mg/L）,不定芽增加3∽5个;不定根诱导培养基为1/2MS＋NAA（0.5mg/L）＋IBA（0.5∽1.0mg/L）,诱导率高达100%;组培苗移植到盛有细沙的花盆中,成活率为100%。
The stems of Celosia argentea L. were used as explants for establishment of high frequency regenera- tion system and study of different hormone combinations on Celosia argentea adventitious bud induction, influence of subculture proliferation and adventitious root induction. The results showed that ： Celosia argentea stem explants induction medium was MS ＋ 6-BA （ 2 mg,/L） ＋ IBA （ 0.5 rag/L） , the induction rate was 100 % ; subculture medium was MS ＋ 6-BA （2mg/L） ＋ IBA （ 0.01 - 0.02 mg/L）, increased 3 - 5 adventitious buds ; adventitious root induc- tion medium was 1/2 MS ＋ NAA（0.5rag/L） ＋ IBA （0.5 - 1.0mg/L） , the induction rate 100% ;tissue culture seedlings were transplanted to pots filled with sand, the survival rate was 100%.
出处
《作物杂志》
CAS
北大核心
2015年第2期49-52,F0003,共5页
Crops
基金
国家自然科学基金(41471270)
广西高校科研项目(201204LX500)
关键词
青葙
再生体系
组织培养
Celosia argentea L.
Regeneration
Tissue culture
