摘要
目的:研究按摩对大鼠骨骼肌急性损伤修复过程中肌卫星细胞激活关键因子神经型一氧化氮合成酶(n NOS)、肝细胞生长因子(HGF)m RNA表达的影响,探讨按摩对于骨骼肌急性损伤修复的作用。方法:将44只SPF级成年雄性SD大鼠按随机抽样法分为3组,正常对照组(A组,n=4),自然恢复组(C组,n=24),按摩组(M组,n=16)。A组不做任何处理,C、M组用改良打击器制备大鼠腓肠肌急性损伤模型。C组不给予按摩,M组于造模后第3天介入按摩治疗。C、M组于造模后第7天、第14天、第21天及第28天取实验动物腓肠肌样本,HE染色观察组织病理改变,实时荧光PCR检测肌卫星细胞中n NOS、HGF m RNA表达量。结果:按摩干预治疗后,M、C组与A组比较,各时间点n NOS、HGF m RNA表达量均高于A组,且具有显著差异(P<0.01);M组与C组比较,在7天、第14天、第21天及第28天n NOS、HGF m RNA表达量M组均高于C组(P<0.01)。M组与C组比较,损伤区域成肌细胞更多,肌丝稠密,肌卫星细胞增殖明显,血供更丰富,坏死组织恢复更快。结论:按摩可有效提高肌卫星细胞中n NOS、HGF m RNA表达水平,激活更多的肌卫星细胞,促进受损骨骼肌的修复再生。
Objective： To investigate the effects of massage on proliferation of muscle satellite cells and the expressions of neuronal nitric oxide synthase（nNOS） and hepatocyte growth factor（HGF） mRNA in acute skeletal muscle in- jury of rats, so that to explore the role of massage on the repair of acute skeletal muscle.
Method： Forty-four SPF adult male SD rats were randomly divided into 3 groups： normal control （group A, n= 4）, self-healing group （group C, n=24）, control group of massage （group M, n=16）. In group A, rats were tak- en care as normal controls; in groups C and M, rats were made to the model of rats with acute muscle injury by the modified traumatizing unit. Group C was not given the treatment, massage was given to group M at the 3rd after modeling. At the 7th, 14th, 21st, and 28th days after modeling, rats gastrocnemius samples were taken in groups C and M respectively, HE staining was employed to inspect pathological changes. Real-time PCR was applied to detect the expressions of nNOS and HGF mRNA in muscle satellite cells.
Result： After massage intervention, the expressions of nNOS and HGF mRNA were higher in groups C and M than in group A at every time point, and there were significant differences （P〈0.01）. Comparing to group C, the expressions of nNOS and HGF mRNA of Group M were higher at the 7th, 14th, 21st and 28th days after modeling （P〈0.01）. HE staining showed that group M had more myoblasts, more myofilaments and more activated muscle satellite cells, blood supply was richer, necrotic tissue recovered faster compared to group C.
Conclusion： Massage can effectively improve the expression levels of nNOS and HGF mRNA in muscle satel- lite cells, and increase the number of activated muscle satellite cells, promote repair and regeneration of the damaged skeletal muscles, provide some basis for improving the level of recovery of body function, and in maximize to reduce the time for returning to training and to participate in the athletic contest after injury.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2015年第3期224-230,共7页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助项目(81273870)
关键词
按摩
肌卫星细胞
神经型一氧化氮合酶
肝细胞生长因子
massage
muscle satellite cell
neuronal nitric oxide synthase
hepatocyte growth factor
