摘要
目的探讨3、7、17号染色体区带特异性探针(CSP3、CSP7、CSP17)和9号染色体p21(9p21)区p16基因位点特异性探针(GLPp16)的荧光原位杂交(fluorescence in situ hybridization,FISH)检测和血清氧化应激参数测定在膀胱移行上皮细胞癌(bladder transitional cell carcinoma,BTCC)中的应用价值,并分析其结果间的相互关系。方法随机选择健康对照30例和BTCC患者188例,运用FISH技术检测其膀胱脱落细胞的CSP3、CSP7、CSP17及GLPp16异常率,同时测定其血清TOS、TAS浓度和OSI值,比较各测定结果在健康人群和BTCC患者间的差异及其与BTCC分期分级的相关性,并进一步分析BTCC患者中CSP3、CSP7、CSP17和GLPp16异常分别和血清氧化应激参数的相关性。结果 BTCC患者与健康对照相比,其膀胱脱落细胞CSP3异常率(47.3%vs.10.0%)、CSP7异常率(52.1%vs.15.0%)、CSP17异常率(41.5%vs.10.0%)、GLPp16异常率(59.0%vs.20.0%)和FISH阳性率(50.5%vs.0.0%)以及其血清TOS浓度[(18.60±3.70).vs.(14.74±1.31)μmol H2O2 Eq./L]、OSI值[(1.33±0.41)vs.(0.89±0.10)]均明显增高(P<0.01),仅其血清TAS浓度[(1.44±0.21)vs.(1.66±0.16)mmol Trolox Eq./L]明显降低(P=0.000)。各探针异常率、FISH阳性率和血清氧化应激参数与BTCC分期之间均不相关(P>0.05)。而与BTCC分级之间,除GLPp16外,CSP3、CSP7、CSP17异常率和FISH阳性率呈明显的Gamma等级相关(r=-0.452、-0.564、-0.519和-0.375,P=0.000),且血清TOS、TAS浓度和OSI值也具有良好的Spearman相关性(r=0.678、-0.311和0.664,均P=0.000)。进一步分析4种染色体异常与3项氧化应激测定结果的相关性显示,CSP3、CSP7、CSP17异常率分别与TOS、OSI结果间均呈正相关(P<0.01),CSP7、CSP17异常率分别与TAS结果间呈负相关(P<0.01);而GLPp16异常率与TOS、TAS、OSI结果间均不相关(P>0.05)。结论 FISH检测膀胱脱落细胞染色体畸变是早期辅助诊断BTCC的有效的、无创性手段,而血清氧化应激参数准确定量可用于病情评估,二者的相关性研究有助于探索染色体畸变的机制以及其与BTCC发生、发展的关系。
Objective To investigate the application value of fluorescence in situ hybridization（FISH）detection for 3, 7, 17 chromosome region-specific probes（CSP3, CSP7, CSP17）and p16 gene locus-specific probe（GLPp16）, and serum oxidative stress states determination in diagnosing bladder transitional cell carcinoma（ BTCC） , and analyze the interrelation of their results. Methods 30 health controls and 188 patients with BTCC were randomly enrolled to be detected the abnormal rate of CSP3, CSP7, CSP17 and GLPp16 in bladder exfoliated cells by FISH, and to be measured serum TOS, TAS concentrations and OSI val-ues at the same time. Afterwards, their deference between healthy controls and patients with BTCC were compared, as well as their correlation with BTCC stages and classifications, and further analyzed the interrelation between serum oxidative stress parame-ters and the probe signal of CSP3, CSP7, CSP17 and GLPp16, respectively. Results In patients with BTCC comparing with healthy controls, the abnormal rate of CSP3（47. 3% vs. 10. 0%）, CSP7（52. 1% vs. 15. 0%）, CSP17（41. 5% vs. 10. 0%）, GLPp16（59. 0% vs. 20. 0%）, and FISH positive incidence（50. 5% vs. 0. 0%） in bladder exfoliated cells were obviously in creased（P〈0. 01）, as well as serum TOS concentrations[（18. 60 ±3. 70） vs （14. 74 ±1. 31）μmol H2O2 Eq. /L]and OSI val-ues（1. 33 ± 0. 41 vs. 0. 89 ± 0. 10）, and only serum TAS concentrations[（1. 44 ± 0. 21） vs. （1. 66 ± 0. 16）mmol Trolox Eq. /L] were obviously reduced（P=0. 000）. With BTCC stages, every chromosomal aberration rate, FISH positive incidence rate and se-rum oxidative stress parameters had no apparent correlations（P〉0. 05）. But with BTCC classifications, except GLPp16, not only the aberration rate of CSP3, CSP7, CSP17, and FISH positive incidence had apparent Gamma rank correlations（r= -0. 452,-0. 564,-0. 519, and -0. 375,P=0. 000）, but also serum TOS, TAS concentrations and OSI values had superior Spearman cor-relation coefficients（r=0. 678、-0. 311 和0. 664, P=0. 000）. By further analyzing the correlation between four chromosomal aberration and three oxidative stress parameters results, it was shown that the abnormal rate of CSP3, CSP7, CSP17 had positive correlations with TOS and TAS results（P〈0. 01）, moreover, the abnormal rate of CSP7 and CSP17 had negative correlations with TAS results（P〈0. 01）, but GLPp16 abnormal rate had no correlations with TOS, TAS and OSI results（P〉0. 05）. Conclu-sionThe chromosomal aberration detection by FISH in bladder exfoliated cells is an efficient and noninvasively means for early aux-iliary diagnosing BTCC, and serum oxidative stress parameters determination may be helpful for disease assessment, the relation-ship study of their results should be contribute to exploring mechanisms of chromosomal aberration, and their relations with the genesis and development of BTCC.
出处
《四川医学》
CAS
2015年第3期303-308,共6页
Sichuan Medical Journal
基金
国家科技部(863计划)课题(编号:2006AA020905)
卫生部科研基金课题(编号:WKJ2007-3-001)
关键词
膀胱移行上皮细胞癌
荧光原位杂交
氧化应激
诊断
bladder transitional cell carcinoma
fluorescence in situ hybridization
oxidative stress
diagnosis
