摘要
应用凝胶电泳和免疫印斑技术对日本血吸虫卵与卫氏并殖吸虫成虫的水溶性抗原(JSEA、PAA)和尿素溶性抗原(JEA-u、PAA-u)进行了抗原蛋白组分比较分析。结果显示:两者的特异性抗原显色主带,JSEA、JEA-u分别有8条和3条,PAA、PAA-u分别有9条和2条;其中JSEA、JEA-u分别有1条和2条,PAA、PAA-u分别有1条和2条对异种抗血清呈现交叉反应显色带。表明两种吸虫的可溶性抗原均存在共同抗原组分。慢性血吸虫病患者血清与并殖吸虫成虫抗原的交叉反应仅见于PAA,而未见于PAA-u,提示PAA-u在避免交叉反应和抗原纯化方面比PAA较为优越。
Using enzyme-linked immunoblot technique (ELIB). the antigenic protein components of water-soluble and urea-scluble antigens of Schistosoma japonicum eggs (JSEA, JEA-U) aad Paragonimus westermani adult worms (PAA, PAA-U) were analysed. The results showed that in both JSEA and JEA-U, 8 and 3 bands of polypeptides could be recogni-zed by sera from patients with schistosomiasis japonica (Sj). In both PAA and PAA-U, there were 9 and 2 bands of polypeptides respectively, which could be recognized by sera from patients with pagumogonimiasis skrjabini (Ps). The components of 36/37kDa in PAA and 20/21 kDa in JSEA were identified to be shared antigenic fractions existing between the two species of trematodes, which gave positive reaction against patient's sera from both S.j. and P.s., while PAA-U caused the reaction only with sera from patients with acute schistosomiasis. PAA-U might be useful to avoid cross reaction in the majority of schistosomiasis patients in the fields.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1991年第1期31-34,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
血吸虫卵
并殖吸虫成虫
免疫印斑
Immunoblot, cross-reactiont Schistosoma japonicum eggs, Paragonimus westermani adult worms, water-soluble antigen, urea-soluble antigen.
