摘要
目的:探讨新型注射型杂合生物材料磷酸钙骨水泥/重组合异种骨粒(injected calcium phosphate cement/recombined bone xenograft,ICPC/RBX)对前交叉韧带(anterior cruciate ligament,ACL)重建术后腱-骨界面愈合的影响。方法:体外制备杂合生物材料,通过扫描电镜观察结构并评测孔隙率,测定凝固时间及体外材料的最大压缩强度。随后将其应用于实验兔ACL重建模型,术后6、12、24周分别通过影像学、组织学及生物力学测试评测腱-骨界面的愈合。结果:与单纯磷酸钙骨水泥(ICPC)制备相比,ICPC/RBX凝固时间无明显改变(P>0.05),最大压缩强度低于ICPC(P<0.05),但孔隙率明显高于ICPC(P<0.05)。ACL重建术后3个测试时间点腱-骨界面新生组织矿化量均显著高于对照组及ICPC组(P<0.05)。组织学观察结果显示,腱-骨界面术后6、12、24周移植肌腱与骨隧道之间间距3组均逐渐缩小,但ICPC/RBX组表现更为显著,且腱-骨界面残余材料较少,新生骨较多。生物力学测试结果进一步证实,ICPC/RBX组腱-骨界面愈合效果优于对照组及ICPC组。结论:具有多孔隙结构且负载BMP的ICPC/RBX杂合生物材料促进腱-骨界面骨生长的作用优于单纯ICPC材料,同时也可以为ACL重建提供初始的协同固定作用,为ACL术后腱-骨界面的早期快速愈合奠定良好基础。
Objective To explore the effect of a new hybrid biomaterial,injected calcium phosphate cement combined with recombined xenograft bone granules（ICPC/RBX）,on the healing of tendon-tobone interface after anterior cruciate ligament（ACL） reconstruction.Methods The hybrid biomaterial was prepared in vitro.The micro-architecture was observed through scanning electron microscope and the porosity rate was evaluated.Then,the setting time and maximum compression load were tested.Thereafter,the hybrid ICPC/RBX was applied to the rabbit ACL reconstruction model.The healing of tendon-to-bone interface was evaluated by imaging,histology and biomechanical test at 6 weeks,12 weeks and 24 weeks after operation.Results Compared with simple injected calcium phosphate cement（ICPC）,the setting time of ICPC/RBX was lengthened and the compression load reduced.The porosity rate of ICPC/RBX was superior to ICPC.The tissue mineral content of new tissue between grafted tendon and bone tunnel increased,and the gap decreased more significantly in the ICPC/RBX treated group as compared with the ICPC treated group and blank control group.Moreover,there were the least debris of biomaterials and the best healing of tendon-to-bone interface in ICPC/RBX treated group as compared with the other groups.Conclusion The bone morphogenetic protein in hybrid and porous ICPC/RBX could enhance the healing of tendon-to—bone interface after ACL reconstruction,providing the initial fixation of the reconstruction.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2014年第12期1166-1173,共8页
Chinese Journal of Sports Medicine
基金
国家体育总局综合攻关项目(10B019)
关键词
前交叉韧带重建
磷酸钙骨水泥
重组合异种骨粒
腱-骨愈合
ACL reconstruction
calcium phosphate cement
recombined xenograft bone granules
tendon-to-bone interface healing
