摘要
目的:探究济生肾气汤对移植后的干细胞在肝硬化大鼠肝脏中归巢定植能力的影响.方法:将分离培养得到的大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)采用DAPI荧光标记,然后将造模成功的肝硬化大鼠随机分为A、B、C、D 4组,每组各15只.A组大鼠单纯采用BMSCs移植治疗;B组采用济生肾气汤中药灌胃联合BMSCs移植治疗;C组采用注射粒细胞集落刺激因子(granulocyte colony stimulating factor,G-CSF)联合BMSCs移植治疗;D组单纯采用济生肾气汤中药灌胃作为对照.4 wk后分别取4组大鼠肝组织做冰冻切片,并于荧光显微镜下观察肝组织中DAPI标记的细胞计数.结果:治疗后,A、B、C、D组大鼠死亡数量分别为2、2、4、0只.A、B、C、D 4组大鼠荧光显微镜(100倍视野)下观察到细胞计数分别为7.31个±1.55个、20.85个±3.21个、15.82个±1.89个、0.00个±0.00个.组间比较显示:B组、C组大鼠荧光显微镜下观察到的细胞计数明显多于A组(P<0.01);B组大鼠荧光显微镜下观察到的细胞计数明显多于C组(P<0.01).结论:济生肾气汤可有效促进移植后的干细胞向肝脏归巢定植且安全性良好.
AIM：To assess the effect of Jisheng Shenqi decoction on homing of stem cells to the liver after transplantation of bone marrow mesenchymal stem cells（BMSCs） in cirrhotic rats.METHODS：BMSCs were labeled with DAPI.Forty cirrhotic rats were randomly divided into four groups（15 cirrhotic rats in each group）：A,B,C and D.Group A underwent BMSC transplantation,group B was given Jisheng Shenqi decoction combined with BMSC transplantation,group C received injection of granulocyte colony stimulating factor（G-CSF） combined with BMSC transplantation,and group D was treated with Jisheng Shenqi decoction.After 4 wk of treatment,liver tissues of rats in each group were taken to observe pathological changes,and cells labeled with DAPI were counted under a fluorescence microscope.RESULTS：After treatment,two rats were dead in each of groups A and B.In group C,4 rats were dead.Under the fluorescence microscope（×100）,the counts of cells labeled with DAPI in the four groups were 7.31 ±1.55,20.85 ± 3.21,15.82 ± 1.89,and 0.00 ± 0.00,respectively.The numbers of cells labeled with DAPI in groups B and C were significantly higher than that in group A（P〈0.01）,and the number in group B was significantly higher than that in group C（P〈0.01）.CONCLUSION：Jisheng Shenqi decoction can effectively promote BMSC homing to the liver after BMSC transplantation,and is safe and feasible.
出处
《世界华人消化杂志》
CAS
2015年第7期1104-1109,共6页
World Chinese Journal of Digestology
基金
广西科学研究与技术开发计划基金资助项目
No.桂科攻1298003-2-10~~
关键词
济生肾气汤
干细胞移植
肝硬化
归巢
动物实验
Jisheng Shenqi decoction
Stem cell transplantation
Liver cirrhosis
Homing
Animal experiment
