摘要
目的探讨急诊重症监护病房(EICU)感染患者的病原菌分布及其耐药性,了解EICU目前流行菌株及药物敏感谱,为临床治疗提供参考依据。方法回顾性分析2012-2013年EICU 520例感染患者送检的标本分离出的阳性菌株,采用法国生物梅里埃公司ALERT 3D60全自动培养仪进行培养,美国BD Phonix 100全自动细菌鉴定仪鉴定菌种及检测耐药性。结果共分离出病原菌348株,革兰阳性菌94株占27.0%,以金黄色葡萄球菌和表皮葡萄球菌居多,革兰阴性菌226株占64.9%,以铜绿假单胞菌、鲍氏不动杆菌、大肠埃希菌及肺炎克雷伯菌居多,真菌28株占8.1%,以曲霉菌属为主;革兰阳性菌对替考拉宁和万古霉素敏感性最好,均无耐药菌株检出,革兰阴性菌对亚胺培南、氨苄西林/舒巴坦较敏感,耐药率<30.0%。结论医院EICU感染病原菌以革兰阴性菌为主,应加强其耐药性的监测,促进临床合理使用抗菌药物。
OBJECTIVE To explore and analyze distribution and drug resistance of the pathogenic bacteria for infections in the emergency intensive care unit（EICU）of our hospital so as to understand the prevalent strains in EICU and provide reference for clinical treatment.METHODS Positive strains of pathogenic bacterial specimens isolated from 520 infected patients in EICU during Jan.2012 to Dec.2013 were retrospectively analyzed.The France BioMerieux ALERT 3D60 automatic culture system was adopted for bacterial culture,and the American BD Phonix100 automated bacterial identification system was adopted for identification of bacteria strain distribution and detection of drug resistance.RESULTS Among the 348 isolated pathogenic bacteria,there were 94（27.0%）strains of gram positive bacteria mainly including Staphylococcus aureus and Staphylococcus epidermidis,226（64.9%）strains of gram negative bacteria mainly including Pseudomonas aeruginosa,Baumanii,Escherichia coli and Klebsiella pneumoniae,and 28（8.0%）strains of fungi mainly including Aspergillus.Gram positive bacteria were most sensitive to teicoplanin and vancomycin,no resistant strains were detected.Gram negative bacteria were sensitive to imipenem and ampicillin/sulbactam with the resistant rate less than 30.0%.CONCLUSIONPathogenic bacteria in EICU of our hospital were mainly gram negative bacteria,and monitoring of their drug resistance should be strengthened to promote reasonable use of clinical antibacterial agents.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第7期1498-1500,共3页
Chinese Journal of Nosocomiology
基金
湖南省科研条件创新专项基金项目(2010TT1008)
