摘要
该实验克隆了毛果杨组蛋白去乙酰化酶基因HDA901的编码序列,并进行生物信息学、亚细胞定位和盐胁迫表达分析。序列分析表明,HDA901开放阅读框为1 245bp,编码1个由414个氨基酸残基组成的蛋白质,等电点为5.77;毛果杨HDA901与其他植物同源蛋白具有一段保守序列,在进化上与拟南芥AtHDA14亲缘关系较近。启动子分析表明,毛果杨HDA901基因启动子序列包含ACE、ABRE、HSE和TC-rich repeats等多个与逆境相关的顺式作用元件。亚细胞定位分析表明,毛果杨HDA901蛋白在细胞核和细胞质中无分布,可能位于线粒体或穿梭于线粒体和叶绿体之间。实时荧光定量PCR结果显示,毛果杨HDA901基因表达受盐胁迫调节,在盐胁迫下,根和茎中HDA901基因表达受抑制;叶中HDA901基因表达受诱导。研究表明,毛果杨HDA901基因参与盐胁迫应答反应。
The Open Reading Frame（ORF）sequence of a histone deacetylase gene,named HDA901,was cloned fromPopulus trichocarpaand its sequence,sub-cellular localization and expression under salt stress were analyzed.The ORF of HDA901 is 1 245 bp,encoding aprotein consisting of 414 amino acids.The isoelectric point of HDA901 protein is 5.77.Sequence alignment and phylogenetic analysis showed that HDA901 shared a conserved domain with the HDACs in other plants and was relatively close to Arabidopsis AtHDA14 in evolution.Promoter sequence analysis showed that the promoter of HDA901 gene contained multiple stress-related cis-acting elements including ACE,ABRE,HSE and TC-rich repeats.Sub-cellular localization analysis showed that HDA901 was localized neither in nucleus nor in cytoplasm,but might be localized in mitochondria and（or）shuttle between mitochondria and chloroplasts.Real-time PCR analysis showed that the expression of HDA901 gene was in response to salt stress.Under the salt stress,the expression of HDA901 was down-regulated in stem and root,while was up-regulated in leaf.These findings indicated that HDA901 was involved in salt stress response.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第3期434-439,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
林木遗传育种国家重点实验室创新项目(2013B06)
国家自然科学基金青年科学基金(31200497)
中国博士后科学基金(2013M540264)
黑龙江省博士后基金(LBH-Z13006)
东北林业大学2014年度大学生科研训练项目(31200497)
关键词
毛果杨
组蛋白去乙酰化酶
克隆
盐胁迫
基因表达
Populus trichocarpa
histone deacetylase
clone
salt stress
gene expression
