摘要
采用心脏取血的方法得到黑龙江草蜥(Takydromus amurensis)、丽斑麻蜥(Eremias argus)、捷蜥蜴(Lacerta agilis)和胎生蜥蜴(Zootoca vivipara)4种蜥蜴的血细胞,用试管稀释法进行血细胞计数,并用Wright染色法对血细胞的成分和形态特征进行分析,使用Leica Q Win软件对各类血细胞进行拍照及测量,测得数据用SPSS 21.0软件进行统计学处理。结果表明:4种蜥蜴血细胞均由红细胞、白细胞和血栓细胞组成,其中白细胞包括嗜中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、淋巴细胞和单核细胞;4种蜥蜴的血细胞在数量与形态学特征方面均存在较明显差别,其中捷蜥蜴白细胞数量最高,红细胞数量及白细胞占血细胞的百分比均较高,红细胞短径最大;丽斑麻蜥红细胞数量较多,但白细胞数量及其占血细胞总数的比例最少,红细胞长径最小,嗜酸性粒细胞最小、单核细胞最大;黑龙江草蜥红细胞、白细胞数量较少,红细胞长短径比值较大,嗜碱性粒细胞直径最大;胎生蜥蜴与黑龙江草蜥相似,但嗜碱性粒细胞直径较小,产生以上差别的原因可能与其进化地位和生态适应相关。
Blood cells of Takydromus amurensis, Eremias argus, Lacerta agilis and Zootoca vivip- ara were collected from hearts, counted using the tube broth method and we analyzed the compo- nents and morphology of blood cells with Wright straining method. All types of blood ceils were photographed and measured with Leica Q Win software. Erythrocyte, leucocyte and thrombocyte were encountered in blood cells. Leucocytes included heterophils, eosinophils, basophils, lym- phocytes and monocytes. Differences existed in blood cell counts, sizes and morphologies be- tween the four species. Lacerta agilis had the highest leucocyte count, high numbers of erythro- cytes, the highest percentage of leucocytes, and the longest short diameter of erythrocytes.Eremias argus had the highest erythrocyte count, fewest leucocytes and lowest percentage count of leucocytes, longest diameter of monocyte, shortest long diameter of erythrocyte and diameter of eosinophil. Takydromus amurensis had fewer erythrocytes and leucocytes, a higher ratio of long to short diameter of erythrocytes, and the longest diameter of basophils, Zootoca vivipara and Takydromus amurensis were morphologically similar, but Zootoca vivipara had shorter diame- ter of basophils. These differences might be related to their evolution status and environment in some degrees.
出处
《野生动物学报》
北大核心
2015年第1期93-97,共5页
CHINESE JOURNAL OF WILDLIFE
基金
国家自然科学基金项目(31172079)
哈尔滨师范大学硕士研究生创新科研项目(HSDSSCX2014-8)
哈尔滨师范大学科技发展预研项目(11XYG-04)
关键词
黑龙江草蜥
丽斑麻蜥
捷蜥蜴
胎生蜥蜴
血细胞
Takydromus amurensis
Eremias argus
Lacerta agilis
Zootoca vivipara
Blood cell
