摘要
目的：探讨酪氨酸激酶抑制剂 Erlotinib 联合放疗对鼻咽癌裸鼠移植瘤的作用。方法将鼻咽癌细胞株 CNE2接种于32只裸鼠后腿皮下，8 d 后肿瘤体积在100～200 mm 3之间，将裸鼠按体积大小随机分为4组：对照组、Erlotinib 组、放疗组和放疗＋Erlotinib 组，每组8只。分组第1天予以照射1次8 Gy，Erlotinib 分组第1天予以灌胃给药，1.6 mg／次，1次／d，连用2周。停药1周后处死动物，测量瘤块体积和重量，采用免疫组织化学法检测各组标本中 EGFR 和 p －EGFR 的表达情况，并进行统计学分析。结果对照组、Erlotinib 组、放疗组、放疗＋Erlotinib 组的瘤重分别为（2.60±1.51）、（1.56±0.67）、（0.71±0.42）、（0.48±0.31）g，放疗＋Erlotinib 组瘤重小于 Erlotinib 及放疗组（P ＝0.026，P ＝0.047）。各组 EGFR 表达无明显差异，p －EGFR 在放疗组明显增强，放疗＋Erlotinib 组阳性表达率最低。结论Erlotinib 通过抑制 EGFR 磷酸化增强了鼻咽癌的放射敏感性。
Objective To investigate the effects of radiotherapy combined with erlotinib on nasopharyngeal carci -noma （NPC） xenografts in nude mice.Methods The NPC cell lines CNE2 were injected s.c.into the hind legs of athy-mic nude mice.When tumor volume was approximately between 100 -200 mm 3 , nude mice were randomly divided into four groups： control, erlotinib, radiation and radiotherapy in combination with erlotinib groups .Radiotherapy was delive-red with 8 Gy in one time on the first day of randomization .Erlotinib was administered by oral gavage （1.6 mg once dai-ly） for 2 weeks.The nude mice were sacrificed 1 week after stopping the treatment , and the tumor size and weight were measured.The expression of epidermal growth factor receptor （EGFR） and phosphorylated EGFR （p -EGFR） were de-tected in histologic sections of xenografts by immunohistochemistry and analyzed by statistical method .Results Erlotinib combined with radiotherapy could significantly slow down tumor growth .Tumor weights in control group , erlotinib group, radiotherapy group and radiotherapy in combination with erlotinib group were （2.60 ±1.51）, （1.56 ±0.67）, （0.71 ± 0.42） and （0.48 ±0.31） g, respectively.Compared with erlotinib group or radiotherapy group , radiotherapy in combina-tion with erlotinib group significantly inhibited the growth of xenografts in nude mice （P =0.026, P =0.047）.There was no significant difference in expression of EGFR among the 6 groups.Conclusion Erlotinib can enhance radiosensitivity of NPC by the inhibition of EGFR phosphorylation .
出处
《广东医学》
CAS
北大核心
2015年第5期674-677,共4页
Guangdong Medical Journal
基金
广州市科技局科技支撑计划项目(编号:2009Z1-E281)
关键词
鼻咽癌
放疗
酪氨酸激酶抑制剂
表皮生长因子受体
nasopharyngeal carcinoma
radiotherapy
erlotinib
epidermal growth factor receptor
