摘要
目的 研究肺炎链球菌脑膜炎水通道蛋白4（AQP4）在脑组织内表达的变化特点,探讨细菌性脑膜炎脑水肿发生的机制.方法 健康3周龄SD大鼠40只,体质量60 -80 g,雌雄不拘,采用随机数字表法随机分为对照组、造模后24 h组、造模后48h组和造模后5d组,每组10只.采用免疫组织化学染色和Westernblot方法分别于造模后24h、48 h、5d后检测脑组织中AQP4蛋白的表达变化.结果 对照组和造模后24h组大鼠无死亡,造模后48h组死亡2只,造模后5d组死亡4只,病死率分别为20％和40％.光镜下可见对照组脑组织AQP4表达呈弱阳性,阳性细胞主要为胶质细胞及血管周围细胞,神经元未见着色.免疫组织化学染色显示模型组AQP4的阳性表达率随水肿程度的增加而增大,与对照组比较,大鼠感染后不同时间段脑组织AQP4的表达均增加,差异有统计学意义（F =91.84,P＜0.01）.Western blot结果显示模型组脑组织AQP4蛋白表达在造模后24h开始升高、48h达高峰、5d时降低,但仍高于对照组,差异有统计学意义（F=14.23,P＜0.01）.结论 细菌感染中枢神经系统后,AQP4的表达增高,且有先增高后下降的规律.提示AQP4参与了感染性脑水肿的发生发展过程.
Objective To investigate the expressions of aquaporin 4 （AQP4） in the bacterial meningitis in rats and to explore the molecular mechanism for brain edema caused by bacterial meningitis.Methods Totally 40 of 3-week-old-Sprague-Dawley healthy rats,body weight 60-80 g,male or female,were divided into a normal control group（n =10）,and infection groups：24 hours after injection（n =10）,48 hours after injection（n =10）,and 5 days after injection（n =10）.The expressions of AQP4 in the brain were detected by immunohistochemistry and Western blot methods respectively after 24 hours,48 hours,5 days of inoculation.Results Mortality rate：no rats in the control group and the infection group after 24 hours were dead.Two rats in the infection group after 48 hours and 4 rats in the infection group after 5 days were dead because of serious sickness,with the mortality rates 20% and 40%,respectively.AQP4 expression was slightly positive under light microscope,and the positive cells mainly surrounded glial cells and blood vessels,while neurons were not dyed.Immunohistochemical staining showed that AQP4 expression in the model group increased with the severity of edema;compared with the control group,the AQP4 expression in the brain tissues increased in different periods after rats were infected,and the differences between groups were statistically significant （F=91.84,P 〈 0.01）.Western blot analysis showed that after the brain received streptococcus pneumoniae injection,expression of AQP4 began to increase in 24 hours after streptococcal injection,and reached to the peak in 48 hours,but decreased in 5 days,but the expression still remained higher than that of the normal control group.Each group had statistically significant difference（F =14.23,P 〈 0.01）.Conclusions Expression of AQP4 in the models with bacterial meningitis may increase initially and decrease later.It suggests that AQP4 plays a protective role during the development of infectious brain edema.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第7期535-537,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
河南省教育厅科学技术研究重点项目（13A320662）
