摘要
目的 :观察中药脊髓康对急性大鼠脊髓损伤后Nogo-A,Ng R基因表达的影响,探讨其对急性脊髓损伤的治疗保护作用及机制。方法:180只雌性SD大鼠,随机分为假手术组、模型组、强的松组及脊髓康高、中、低剂量组,每组30只。假手术组:切除T10节段椎板,不损伤脊髓;其余组采用改良Allen法建立脊髓损伤模型。强的松组:术后至处死前每日灌胃强的松0.06 g/kg,首次在术后30 min给药。脊髓康高、中、低剂量组:在同一时间分别给予脊髓康,每日50、25、12.5 g/kg。术后3、7、14 d分批处死动物,取损伤节段脊髓9例,分别利用Western Blot及Real-time PCR检测脊髓组织中Nogo-A、Ng R蛋白及m RNA表达水平。结果:假手术组在各时间点Nogo-A及Ng R的表达维持在基础水平。与模型组比较,各时间点假手术组、强的松组及脊髓康中剂量组Nogo-A、Ng R蛋白表达差异均有统计学意义,脊髓康高、低剂量组差异无统计学意义。术后3 d,各治疗组Nogo-A、Ng R m RNA水平均低于模型组;术后7 d,模型组Nogo-A、Ng R m RNA水平升至最高,14 d时有所下降,但仍高于其他治疗组,其中强的松、脊髓康中剂量组效果最显著,两者之间差异无统计学意义。结论:在Allen急性大鼠脊髓损伤后,脊髓康能调节Nogo-A及Ng R基因表达,激活Nogo-Ng R信号通路,促进脊髓损伤后神经细胞再生。
Objectlve：To study the effects of Jisuikang （脊髓康） on Nogo-NgR gene expression, and to explore the pro- tective effects and mechanism of Jis uikang （脊髓康） on spinal cord injury in rats. Methods：One hundred eighty female rats were randomly assigned to 6 groups （30 rats per group）. Sham group ：T10 lamina was resected only and spinal cord was untreat- ed. Model group：spine cord injury （SCI） was created with a modified impinger of Allen＇s by impacting on the Tl0 spinal cord. Prednisolone group ： Prednisolone （0.06 g/kg） was given by intragastric administration at a time interval of 24 hours after oper- ation. The Jis uikang （ 脊髓康） high, moderate and low dose groups ： Jis uikang （ 脊髓康） was supplied with different dose （50 g/kg,25 g/kg, 12.5 g/kg） by intragastric administration in rats after operation,for the first time at 30 rain after surgery. Ani- mals were killed 3,7,14 days after surgery. The expression levels of Nogo-A and NgR were observed by Western Blot and Real- time PCR. Results ：The expression of Nogo-A and NgR was at the basic level at all time points in sham group. Compared with model group, the protein expression levels of Nogo-A and NgR in sham,prednisolone ,Jisuikang （脊髓康） moderate dose groups were statistically significant at all time points （P〈0.05）. No difference was found in Jisuikang （脊髓康） high and low dose groups （P〉0.05）. Three days after surgery,the mRNA levels of Nogo-A and NgR in treatment group were significantly lower than that in model group （P〈0.01） ;7 days after surgery, Nogo-A and NgR mRNA expression were dramatically upregu- lated and peaked; 14 days after operation, the expression was decreased, but still significantly higher than that in other treat- ment groups （P〈0.01）. Prednisolone and Jisuikang （脊髓康） moderate dose groups showed the most significant effects among all groups,but there was no statistically significant difference between two groups （P〉0.05）. Conclusion：The decoction Jis uik ang （脊髓康 ） can promote the nerve cell regeneration by regulating Nogo-A and NgR gene expression, activating Nogo- NgR signaling pathways after acute spinal cord injury.
出处
《中国骨伤》
CAS
2015年第3期235-239,共5页
China Journal of Orthopaedics and Traumatology
基金
江苏省自然科学基金资助项目(编号:BK2011180)
江苏省2013年度普通高校研究生科研创新计划项目(编号:CXZZ13-0614)~~
关键词
脊髓损伤
基因表达
中草药
活血通络
Spinal cord injuries
Gene expression
Drugs,Chinese herbal
Activate blood dredging collaterals
