摘要
目的 研究血清白细胞介素6（IL-6）、白细胞介素8（IL-8）和肿瘤坏死因子α（TNF-α）的检测对于诊断活动性肺结核患者的临床意义。 方法 2012年9月至2013年6月在苏州市第五人民医院诊断为初治活动性肺结核的患者71例（活动性肺结核组）和既往明确诊断为肺结核，经过正规方案抗结核完成治疗后，同期门诊随访复查的陈旧性肺结核患者21例（对照组），采用酶联免疫吸附法（ELISA）检测两组患者血清中IL-6、IL-8和TNF-α水平的变化情况，进行对比分析。应用SSPS 13.0统计软件进行统计学分析，计量资料呈非正态分布，采用中位数和上下四分位数表示，组间比较采用Wilcoxon秩和检验，计数资料采用卡方检验，以P〈0.05为差异有统计学意义。 结果 （1）活动性肺结核组和对照组血清中TNF-α中位数分别为11.5 pg/ml和8.8 pg/ml，四分位数间距（P25，P75）分别为（9.6 pg/ml，14.7 pg/ml）和（7.6 pg/ml，11.2 pg/ml），两组间比较采用Wilcoxon秩和检验，差异有统计学意义（U=369.5，P〈0.001）。（2）活动性肺结核组和对照组血清中IL-6中位数分别为7.8 pg/ml和2.0 pg/ml，四分位数间距（P25，P75）分别为（3.1 pg/ml，15.9 pg/ml）和（1.5 pg/ml，5.4 pg/ml），两组间差异有统计学意义（U=389.5，P=0.001）。（3）活动性肺结核组和对照组血清中IL-8中位数分别为8 pg/ml和8 pg/ml，四分位数间距（P25，P75）分别为（5 pg/ml，15 pg/ml）和（6.5 pg/ml，13 pg/ml），两组间差异无统计学意义（U=731.5，P=0.896）。 结论 血清TNF-α和IL-6水平在活动性肺结核的发展过程中变化较大；检测患者的TNF-α和IL-6分泌水平或许有助于判断疾病的进程。
Objective To explore the clinical significance of detection of serum IL-6,IL-8 and TNF-α in patients with active tuberculosis（TB）. Methods Seventy-one new TB patients （active TB group） and 21 TB patients who was diagnosed as TB and finished treatment （control group） in The Fifth People’s Hospital of Suzhou from September 2012 to June 2013 were enrolled. The serum contents of IL-6,IL-8 and TNF-α were examined using ELISA assay. SPSS 13.0 was used for data analysis. Median and interquartile range were used to describe numerical data. Wilcoxon rank testing was used for comparison between groups and Chi-square test was applied to compare categorical data. P〈0.05 was considered statistically significant. Results （1） The medians of TNF-α in active TB group and control group were 11.5 pg/ml and 8.8 pg/ml respectively, and the interquartile ranges （P25,P75） were （9.6 pg/ml, 14.7 pg/ml） and （7.6 pg/ml, 11.2 pg/ml）, respectively. There was a significance difference between the two groups （U=369.5, P〈0.001）. （2） The medians of IL-6 in active TB group and control group were 7.8 pg/ml and 2.0 pg/ml, respectively, and the interquartile ranges （P25,P75） were （3.1 pg/ml, 15.9 pg/ml） and （1.5 pg/ml, 5.4 pg/ml）, respectively. The difference between the two groups was statistical significant （U=389.5, P=0.001）. （3） The medians of IL-8 in active TB group and control group were 8 pg/ml and 8 pg/ml, respectively, and the interquartile ranges （P25,P75） were （5 pg/ml, 15 pg/ml） and （6.5 pg/ml, 13 pg/ml）, respectively. There was no significance difference between the two groups （U=731.5, P=0.896）. Conclusion The serum TNF-α and IL-6 in the development of active TB changes in a wide range, and the detection of TNF-α and IL-6 may help to determine the progress of active TB.
出处
《中国防痨杂志》
CAS
2015年第4期344-347,共4页
Chinese Journal of Antituberculosis
基金
2012年苏州市科技局科技支撑计划（SS201241）
