摘要
目的：利用小鼠肝癌细胞株H22细胞肝脏原位种植法建立BALB／c小鼠原位移植肝癌模型，为研究肝脏肿瘤奠定基础。方法取新鲜小鼠腹腔传代的H22细胞，生理盐水洗涤2次，调细胞浓度为1×10^7／ml，与Matrigel按4∶1比例混合，用10μl微量进样针在开腹直视下沿肝叶长轴种植于BALB／c小鼠的肝左叶，每只5μl，逐层关腹，常规饲养2周后，处死小鼠观察肿瘤形成情况，检测小鼠肝脏原位癌的形成率及小鼠存活率，形态学和病理学方法鉴定造模效果。结果肉眼可见小鼠肝脏出现单个灰白肿瘤结节，病理学方法证实为肝癌，小鼠存活率和成瘤率均为95％，肿瘤大小均匀。结论成功建立了一种稳定、简单可行的BALB／c小鼠原位移植肝癌模型。
[ ABSTRACT] Objective Using a mouse hepatoma cell line H 22 cells liver orthotopic implantation method to establish the BALB /c mouse liver orthotopic implantation cancer model ,which lays a foundation for the study of liver tumors .Methods Using normal saline wa-shes the fresh mouse Intraperitoneal passaged H 22 cells two times,cell concentration was adjusted to 1 ×10^7/ml,and mixed with the Matrigel according to the proportion of 4∶1,then injected 5μl cell suspention to the left lobe of the liver along the long axis with 10μl micro needle in the open abdominal cavity with orthophoria and closed abdomen layer by layer ,regular feeding for two weeks before the mice were killed ,then the tumor formation rate and the survival rate of mice were detected ,morphological and pathological methods were used to identify the effect of the tumor formation .Results A single gray mouse liver tumor nodules was observed by the naked eyes in the mouse liver ,pathology method identified it as a liver cancer ,the rate of mouse survival and Tumor formation were all 95%,the tumor size was uniform .Conclusion A sta-bile,simple and feasible BALB/c orthotopic implantation liver cancer model was successfully established .
出处
《潍坊医学院学报》
2015年第1期1-3,25,共4页
Acta Academiae Medicinae Weifang
基金
山东省教育厅资助课题(课题编号:J10LF62)
山东省卫生厅资助课题(课题编号:2013WS0287)
山东省高等学校优秀骨干教师国际合作培养项目资助课题
关键词
H22细胞
原位移植肝癌模型
小鼠
1-122 ceils ~ Orthotopic transplanted liver cancer model
Mice
