摘要
湿地土壤中最主要的有机磷形态是肌醇磷酸盐,占有机磷总量的60%左右,而植酸酶作为催化水解肌醇磷酸盐的主要酶类,在整个有机磷体系转化过程中起着不可或缺的作用。采用室内培养的方法,研究了添加外源植酸酶对湿地土壤各有机磷组分含量变化的影响规律,同时也研究了在添加外源植酸酶后,湿地土壤各有机磷组分含量随时间延长的变化规律。结果表明,在温度,水分等条件适宜的情况下向湿地土壤中添加植酸酶可以在短期内提高土壤有机磷的有效性,使稳定性较高的有机磷向活性较高的有机磷转化。经植酸酶处理的土壤活性有机磷和中活性有机磷含量均高于对照组,且随植酸酶添加量增加以及培养时间的推移呈递增趋势。在第75天培养结束时,高、中、低3组植酸酶处理土壤的活性有机磷质量分数分别增加了173.05%、158.16%和122.75%,中活性有机磷质量分数增加了84.22%、68.7%和53.92%。此外,添加植酸酶处理的湿地土壤稳定性有机磷含量均低于对照,高、中、低3组植酸酶处理土壤的中稳定性有机磷质量分数减少了62.81%、37.31%和26.14%,高稳定性有机磷质量分数减少了94.71%、87.66%和79.52%,且随着植酸酶含量的增加以及培养时间的推移呈递减趋势。高、中、低3组植酸酶处理土壤中的总有机磷质量分数分别比对照组低54.03%、42.53%和35.43%。说明在植酸酶作用下,土壤中存在有机磷向无机磷转化的过程。因此通过添加外源植酸酶可以促进稳定性有机磷向活性有机磷转化,提高了土壤有机磷的有效性,使其更容易被植物吸收利用,从而保障了湿地磷循环系统的稳定性和高效性。
Inositol phosphates were as the main form of wetlands soil organic phosphorus, comprising up to 60%of organic P, and it can be catalyzed by phytases into phytic acid and inorganic phosphorus. Wetlands soil incubation was carried out to study the influ-ences of organic phosphorus fractions and quantity in different periods after application of outside source of phytase. The results were concluded：the effectiveness of organic P can be improved by phytases under the appropriate conditions for short periods. The outside source of phytase increased the contents of labile, moderately labile organic P in soil which is higher than CK. During the cultivation of the 75 days periods, the labile organic P contents of experimental soil increased by 173.05%, 158.16%, and 122.75%, respectively, and the contents of moderately labile organic P increased by 84.22%, 68.7%and 53.92%, respectively, after each treat-ment. In addition, the contents of moderately resistant and highly resistant organic P when added phytases were lower than that of CK. The moderately resistant organic P contents of experimental soil decreased by 62.81%, 37.31%and 26.14%, respectively, and the contents of highly resistant organic P decreased by 94.71%, 87.66%and 79.52%, respectively, and it show a downward trend with increase of phytases contents and time passing. The contents of total organic P also decreased by 54.03%、42.53%and 35.43%, re-spectively than CK, under the effects of phytases. Therefore, Phytases could promote the changes of organic P fractions and increase the availability of soil organic P. To make it more easily to be absorbed by the plant, so as to guarantee the stability and high effi-ciency of the wetland phosphorus cycle system.
出处
《生态环境学报》
CSCD
北大核心
2015年第2期250-254,共5页
Ecology and Environmental Sciences
基金
国家自然科学基金项目(51279004)
人事部留学基金委择优资助项目(优秀项目类)
关键词
植酸酶
湿地土壤
有机磷组分
野鸭湖湿地
phytase
wetlands soil
organic phosphorus frictions
Yeyahu wetland
