摘要
目的初步研究淫羊藿苷对人牙周膜细胞（human periodontal ligament cells,h PDLC）增殖以及在内毒素（Lipopolysaccharide,LPS）作用下淫羊藿苷对h PDLC白细胞介素6（IL-6）表达的影响。方法组织块法原代培养并鉴定h PDLC,采用MTT方法检测淫羊藿苷（0、10-5、10-6、10-7、10-8、10-9、10-10mol/L）作用下h PDLC增殖的变化;利用PCR、ELISA方法检测淫羊藿苷对在LPS刺激下的人牙周膜细胞IL-6分泌的情况。结果一定浓度范围下（10-6-10-7mol/L）淫羊藿苷对h PDLC的增殖具有积极作用;在10μg/m L LPS作用下h PDLC的IL-6表达增多、活性增强,加入10-6mol/L淫羊藿苷处理后,对此加强效果有一定的抑制作用。结论在一定浓度范围作用下淫羊藿苷可促进h PDLC的增殖;同时,淫羊藿苷对LPS导致h PDLC的IL-6表达增强有一定的抑制作用。
Objective To study the effects of icariin（ICA）proliferation of huaman periodontal ligament cells（h PDLC）and the interleukin-6（IL-6）activity inhibited by LPS. Methods h PDLC were cultured with tissue cultivation in virto and stimulated with icariin with different concentrations（0、10-5、10-6、10-7、10-8、10-9、10-10mol/L）for 48 hours. The proliferation of h PDLC was detected by MTT assay. The activity and the expression of IL-6 of h PDLC treated with icariin at the concentration of 10-6mol/L inhibited by LPS was separately determined by RT-PCR and ELISA. Results icariin had a dose-dependent positive influence on the proliferation of the h PDLC in a suitable concentration range from 10-6-10-7mol/L. At the same time, the expression of IL-6 of h PDLC was remarkably enhanced treating with 10μg/ml LPS and was inhibited icariin at the concentration of 10-6mol/L. Conclusion Icariin has the positive effect on the proliferation of h PDLC and the inhibiting effect on IL-6 activity improved by LPS.
出处
《现代口腔医学杂志》
CAS
CSCD
2015年第2期80-83,共4页
Journal of Modern Stomatology
