摘要
观察从中华眼镜蛇(Najanajaatra)蛇毒中分离纯化的眼镜蛇因子对油酸型呼吸窘迫综合征的保护作用。方法利用质谱仪、呼吸流量计和压力换能器分别连续监测麻醉狗的主要呼吸功能参数 ,实验后取肺组织作病理学检查。结果预先用足量眼镜蛇毒因子使体内补体耗竭95 %以上 ,可显著减轻油酸引起的肺功能障碍 ,组织学检查发现在透明膜形成、肺泡内出血及肺小动脉栓塞等方面均有显著改善。结论活化的补体在油酸引起的肺损伤的发展过程中发挥着重要作用 ,眼镜蛇毒因子通过耗竭血清补体可抑制呼吸窘迫综合征的发展目的观察从中华眼镜蛇(Najanajaatra)蛇毒中分离纯化的眼镜蛇因子对油酸型呼吸窘迫综合征的保护作用。方法利用质谱仪、呼吸流量计和压力换能器分别连续监测麻醉狗的主要呼吸功能参数 ,实验后取肺组织作病理学检查。结果预先用足量眼镜蛇毒因子使体内补体耗竭95 %以上 ,可显著减轻油酸引起的肺功能障碍 ,组织学检查发现在透明膜形成、肺泡内出血及肺小动脉栓塞等方面均有显著改善。结论活化的补体在油酸引起的肺损伤的发展过程中发挥着重要作用 。
Aim To study the protective effect of cobra venom factor (CVF), separated from the venom of Naja naja atra, on respiratory distress syndrome (RDS) induced by oleic acid. Methods The main parameters of respiratory function in anaesthetized dogs were continually monitored by mass spectrometer, pneumo-tachograph and pressure transducers. The lung tissues were taken immediately after the experiment for histological examination. Results CVF, given 20 h before the dosage depleting more than 95% of the complements, significantly attenuated oleic acid-induced pulmonary dysfunction. Histological examination showed improvement of hyaline membrane formation, alveolar haemorrhage and pulmonary arteriolar thrombosis. Conclusion Complement plays an important role in the development of lung injury induced by oleic acid. The depletion of serum complements by CVF inhibits the development of RDS.
基金
中华医学基金会(CMB)资助
国家自然科学基金(No.39270782)资助
关键词
蛇毒因子
眼镜蛇
油酸
呼吸窘迫综合征
补体
实验研究
cobra venom factor,
Naja naja atra
oleic acid
respiratory distress syndrome
complement
