摘要
目的：探讨贯叶连翘提取物（ HPL ）对刀豆蛋白 A （ ConA）诱导的小鼠急性免疫性肝损伤的保护作用。方法60只ICR小鼠随机分为正常组、模型组、HPL低剂量组（25 mg/kg）、HPL中剂量组（50 mg/kg）、HPL高剂量组（100 mg/kg）和地塞米松组（2．5 mg/kg）。灌胃给予小鼠不同剂量HPL10 d后,采用尾静脉注射ConA 20 mg/kg制备急性免疫性肝损伤模型。8~12 h后称重,处死小鼠,检测肝脾指数；检测血清中丙氨酸氨基转移酶（ ALT）、门冬氨酸氨基转移酶（ AST）、γ-谷氨酰转肽酶（γ-GT）活性和胆红素、球蛋白含量；检测肝组织中超氧化物歧化酶（ SOD）活性、丙二醛（ MDA）和Toll样受体4（ TLR4）含量；光镜下观察肝组织病理学变化。结果与模型组比较,HPL显著降低小鼠肝脾指数、血清中ALT、AST、γ-GT活性和胆红素、球蛋白含量；降低肝组织中MDA含量和TLR4表达,提高SOD活性；减轻肝组织病理损伤程度。结论 HPL对小鼠急性免疫性肝损伤有明显的保护作用。
Objective To investigate the protective effect of Hypericum Perforatum. L （ HPL） on concanavalin A （ Con A）-induced acute immunological liver injury in mice. Methods Sixty ICR mice were randomly divided into six groups：normal group, model group, HPL （25, 50, 100 mg/kg） groups and positive control group （ dexam-ethasone, 2. 5 mg/kg）. The model of mice acute immunological liver injury was induced by tail intravenous injec-ting with ConA （20 mg/kg） on day 10 after treatment with different concentrations of HPL. After 8 to 12 h, mice were weighed and sacrificed. The liver and spleen index were calculated, the level of ALT, AST, γ-GT, TBIL, GLB in serum and SOD, MDA, TLR4 in liver tissues were tested. Additionally,the hepatic pathological changes were observed. Results Compared with model group, the liver and spleen index, the level of ALT, AST, γ-GT, TBIL, GLB in serum and the content of MDA and TLR4 in liver tissue were decreased, the SOD activity was in-creased in HPL groups. Moreover, the hepatic pathological injury was obviously alleviated after treatment with HPL. Conclusion HPL has obviously protective effects on acute immunological liver injury mice, which might be associated with its ability of decreasing TLR4 expression in liver tissue.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第4期477-481,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:1408085MH178)
