摘要
分别将大肠杆菌、枯草芽孢杆菌、白僵菌及其细胞壁物质脂多糖、肽聚糖和甘露聚糖与家蚕血液在毛细针管中混合,体外观察外源物诱导家蚕血液黑化反应的免疫防御作用。结果表明3种微生物及其胞壁物质均能诱导家蚕血液发生黑化反应,与家蚕血液混合后的大肠杆菌数目随混合时间延长及血液黑化程度加剧而下降。说明家蚕血液黑化反应与昆虫其他的免疫应答反应相同,能被微生物以及相关分子诱导和启动,发挥免疫防御作用,并且大肠杆菌在加入昆虫黑色素合成前体——多巴和多巴胺的培养基中生长繁殖受到抑制的实验结果,也是该结论的佐证。
After silkworm（ Bombyx mori） haemolymph was mixed with three types of microorganisms（ Escherichia coli,Bacillus subtilis and Beauveria bassiana） and their cell wall ingredients（ lipopolysaccharide,peptidoglycan and mannan） in capillary respectively,the melanization of silkworm haemolymph was observed in vitro. The results showed that these three microbes and their cell wall ingredients can all induce haemolymph melanization in silkworm. The number of bacteria decreased with the increasing of mixing time and the degree of melanization after mixed with Escherichia coli.The above results showed that silkworm haemolymph melanization was identical to other immune responses of insects. It has an immunologic defensive function,and can be induced and initiated by microorganisms and related-molecules. This conclusion was further confirmed by that dopa and dopamine,the precursors in melanin synthesis pathway,can inhibit the growth and proliferation of Escherichia coli.
出处
《蚕业科学》
CAS
CSCD
北大核心
2015年第2期278-285,共8页
ACTA SERICOLOGICA SINICA
基金
重庆市自然科学基金重点项目(No.2013jj B80004)
重庆市研究生科研创新项目(No.CYS14048)
关键词
家蚕血液
黑化反应
微生物
免疫应答
体外实验
Haemolymph of Bombyx mori
Melanization
Microorganism
Immune response
In vitro experiment
