摘要
目的:观察不同环境和居住方式对快速老化(SAMP8)小鼠认知功能和海马CA1区N-甲基-D-天门冬氨酸受体1(NMDAR1)的影响,探讨环境及社会支持系统提高认知功能延缓衰老的可能机制。方法:健康雄性6月龄SAMP8小鼠32只,随机分为丰富环境+群居组(A组)、丰富环境+独居组(B组)、普通环境+群居组(C组)和普通环境+独居组(D组)。环境和居住方式干预2个月后通过Morris水迷宫实验测定小鼠的认知功能,免疫组织化学方法测定海马CA1区NMDAR1免疫反应性的表达。结果:Morris水迷宫结果:随训练天数增加,各组潜伏期均有缩短;A组潜伏期明显短于其他各组(P<0.05),空间探索试验中跨越平台次数最多(P<0.05)。B组和C组两者相比无明显差异,但都优于D组(P<0.05)。抗NMDAR1免疫组化染色结果:D组免疫反应产物表达最少,染色最淡;A组可见NMDAR1蛋白免疫反应产物表达较多,细胞排列紧密,染色较深。光密度值分析显示A组光密度值最高,D组光密度值最低。结论:丰富环境和群居能改善SAMP8小鼠的学习记忆及认知功能;增加海马CA1区NMDAR1的蛋白含量;但环境和居住方式是SAMP8小鼠认知功能和海马的独立性保护因素,二者无交互作用。
Objective： To observe the influence of different environment and way of living on cognitive function and Nmethyl- D- aspartate- receptor 1（NMDAR1） in hippocampal CA1 area of senescene accelerated mouse prone 8（SAMP8） mice, and to discuss the possible mechanisms of environment and social support systems to improve cognitive function and anti-aging.Method： Thirty-two 6-month-old male SAMP8 were randomly divided into enriched environment and living in groups group（A）, enriched environment and living alone group（B）, general environment and living in groups group（C）, general environment and living alone group（D）. After 2 months intervention, measure the cognitive function by Morris water maze（Mwm） test, and determine the expression of NMDAR1 immune reactivity in hippocampal CA1 area by immunohistochemical method.Result： In the MWM test, the escape latency of group A was significantly shorter than that in the other groups（P〈0.05）, and the times of span flat roof was the most（P〈0.05）. There was no obvious difference between groups B and C, both better than that of group D（P〈0.05）. With anti-NMDAR immunohistochemistry,the expressions of immune reaction products were the least in group D, neurons arranged loosely, and lightlydyed; while the expression products in group A were much more than other groups, neurons arranged closely and were deeply dyed. Analysis of optical density value showed the optical density value in group A was the highest, while in group D, it showed a minimum set of optical density value.Conclusion： Rich environment and social could improve the cognitive function of SAMP8 mice, increase NMDAR1 protein expression in hippocampal CA1 area. The effects of rich environment and social（increase social interaction） on protecting hippocampal neurons and improving the cognitive functions of SAMP8 mice were independent, no interaction between them.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2015年第4期329-333,343,共6页
Chinese Journal of Rehabilitation Medicine
关键词
丰富环境
居住方式
快速老化
小鼠
认知功能
N-甲基-D-天门冬氨酸受体1
enriched environment
way of living
senescence accelerated
mouse prone 8
mouse
cognitive function
N-methyl-D-aspartate receptor 1
