摘要
目的对崇左市2013年手足口病实验室检查结果进行分析,了解手足口病病原学特征,为实验室诊断手足口病提供参考。方法采集2013年全市各地送检的428例手足口病患者的肛拭子、咽拭子、疱疹液等标本432份,应用Real Time RT-PCR技术检测样本中的肠道病毒71型(Human enterovirus 71,EV 71)、柯萨奇病毒A组16型(Coxsachie virus A16,Cox A16)。结果 428例手足口病患者的432份各类样本中,检测到EV 71病毒特异性基因片段阳性41份、Cox A16特异性基因片段阳性67份、无标本检测出EV 71和Cix A16混合感染。428例手足口病患者中,Cox A16的检出率较EV 71高,检出率分别为15.65%(67/428)和9.58%(41/428);13例手足口重症患者中4例均为EV 71感染患者、1例为Cox A16。患者人群男性高于女性(1.84:1),差异无统计学意义(χ2=0.001,P>0.05)。肛拭子标本的病毒检出率与咽拭子标本差异无统计学意义(χ2=1.344,P>0.05)。结论 Cox A16是2013年崇左市手足口病的优势病原体,EV 71是手足口重症患者的主要毒株类型。
Objective To understand the pathogen characters of hand-foot-mouth disease（ HFMD） and provide reference for laboratory diagnosis of HFMD. Methods A total of 432 samples including throat swabs,Herpes fluid,anal swab,from 428 patients all over Chongzuo city in 2013. Nucleic acids of 2 types of viruses of human enterovirus 71（ EV71） and Coxasckie virus A16（ CVA16） were detected by real time RT-PCR method both in original samples. Results In 432 samples from the patients,41 were EV71 positive,67 were CVA16,no specimen with combined infection of EV71 and CVA16 was detected. In the 428 HFMD cases,the EV71 positive rate was 9. 58 %（ 41 /428）,and the CVA16 positive rate was 15. 65 %（ 67 /428）. 13 cases of HFMD in severs cases,4 cases were EV71 infecton,1 case of CVA16. The proportion of male and female in the cases of HFMD was 1. 84： 1,no statistically significant difference（ χ2= 0. 001,P〉0. 05）.No statistical differences was anal swab specimens of virus detection rate and the pharyngeal swab specimens（ χ2= 1. 344,P〉0. 05）. Conclusions CVA16 was the dominant pathogen of the HFMD in Chongzuo city in2013,and the EV71 was main strains causing HFMD of severe cases.
出处
《医学动物防制》
2015年第4期384-386,共3页
Journal of Medical Pest Control
