摘要
埃博霉素B是一种由粘细菌纤维堆囊菌产生的新型抗肿瘤药物,目前埃博霉素B的发酵生产主要采用原位分离发酵工艺,即在发酵过程中将产生的埃博霉素B从发酵液中不断地吸附到大孔树脂上.本文以吸附有有效成分埃博霉素B的大孔树脂XAD-16为研究对象,确定出其最适解吸附条件.通过实验得出:重复3次解吸已基本将埃博霉素B完全解吸到溶剂中,80%甲醇溶液的解吸附能力最适,解吸附时间为120min,解吸附pH为6.0,最适解吸附的转速为200r/min.该条件的确定无论对实验室还是对于工业化条件下的埃博霉素的提取都具有一定的指导意义.
Epothilone B is a novel anti-tumor drugs produced by myxobacteria sorangium cellulosum.Now,epothilone B is produced through in situ separation of fermentation that epothilone B is adsorbed on macroporous resin continuously from fermentation liquor in the fermentation process.In this paper,macroporous resin XAD-16 with effective components of epothilone B were the main study object,and the best desorption conditions were determined.The results were as follows：the epothilone B had been basically complete desorbed to the solvent when desorption times was 3,80% aqueous methanol solution had the best adsorption ability,the best adsorption time was 120 min,the best adsorption pH was 6.0,the adsorption speed was 200r/min.The conclusion had certain guiding significance both for laboratory and for industrialized extraction of epothilone.
出处
《陕西科技大学学报（自然科学版）》
2015年第2期121-125,共5页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金项目(20906058)
陕西科技大学学术骨干培育计划项目(XSG2010009)
关键词
埃博霉素B
大孔树脂
解吸附
高效液相色谱法
epothilone B
macroporous resin
desorption
high performance liquid chromatography
